[Histonet] Re: Histonet Digest, Vol 97, Issue 28

Fri Dec 23 23:18:20 CST 2011

Andria,
I purchased my HSV I/II from Cell Marque (RTU).  They work very well
with Dako Plus & Leica Bonds.
These antibodies does NOT required antigen retrieval.

Dako Plus:
1) Deparafinize & hydration
2) Quench with Dako Dual Endogenous Block (5')
3) Incubate with 1st ab (HSV I or HSV II) (60')
4) Incubate with 2nd ab, Dako Envision+ M/R-HRP (30')
5) Envision+ DAB (5')
6) Mayer Hematoxylin (1')
7) TBST (30")
8) Dehydrate & Mount

Leica Bonds (Refine DAB Kit);
1) Use Protocol "F"
2) Incubate 1st ab with NO AR "----"(Fill Open Vial with Cell Marque's
HSV I/II)
3) DAB with DAB Enhancer

Good Luck!

Madeleine
Supervisor Pathology - IPOX & Histology
El Camino Hospital
Mountain View, CA 94040

On Fri, Dec 23, 2011 at 10:00 AM,
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
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>   1. Happy Holidays  (Brian-Prometheus)
>   2. AUTO: Mills,      Demaris  is out of the office. (returning
>      01/03/2012) (Demaris.Mills <@t> leica-microsystems.com)
>   3. gram controls (Webb, Dorothy L)
>   4. Antibody to Cryptococcus (Richard Cartun)
>   5. HSV1 and HSV2 (Evans, Andria B)
>
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> Message: 1
> Date: Fri, 23 Dec 2011 10:42:39 -0500
> From: "Brian-Prometheus" <brian <@t> prometheushealthcare.com>
> Subject: [Histonet] Happy Holidays
> To: <histonet <@t> lists.utsouthwestern.edu>
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> Seasons Greetings from all of us here at Prometheus Healthcare!
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>
> Brian Feldman
>
> Principal
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> Prometheus Healthcare
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> Office 301-693-9057
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> Message: 2
> Date: Fri, 23 Dec 2011 10:01:29 -0600
> From: Demaris.Mills <@t> leica-microsystems.com
> Subject: [Histonet] AUTO: Mills,        Demaris  is out of the office.
>        (returning 01/03/2012)
> To: histonet <@t> lists.utsouthwestern.edu
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> I am out of the office until 01/03/2012.
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> I will be out of the office on annual leave from Dec 21 through Jan 3.
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> Note: This is an automated response to your message  "Histonet Digest, Vol
> 97, Issue 27" sent on 12/23/2011 9:39:17 AM.
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> This is the only notification you will receive while this person is away.
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> Message: 3
> Date: Fri, 23 Dec 2011 10:51:44 -0600
> From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
> Subject: [Histonet] gram controls
> To: "'histonet <@t> lists.utsouthwestern.edu'"
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> I remember giving a couple of chicken livers to micro and they incubated them overnight with gram +/-. I then cut those pieces smaller and processed them in separate cassettes.  This process has owrked real well for us.  Was in the Journal of Histotechnology but cannot remember which issue a few years back.
>
> Dorothy Webb
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> Message: 4
> Date: Fri, 23 Dec 2011 12:34:51 -0500
> From: "Richard Cartun" <Rcartun <@t> harthosp.org>
> Subject: [Histonet] Antibody to Cryptococcus
> To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
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> Unfortunately, we have used the last micro-liter of our Cryptococcus antibody that we obtained from DAKO years ago.  Since DAKO no longer sells this antibody I am wondering if anyone is using another commercially-available antibody to label Cryptococcus in formalin-fixed tissue with immunohistochemistry?
>
> Happy Holidays to all my friends and colleagues working in the field of Histology.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
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> ------------------------------
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> Message: 5
> Date: Fri, 23 Dec 2011 12:48:37 -0500
> From: "Evans, Andria B" <aevans3 <@t> lghealth.org>
> Subject: [Histonet] HSV1 and HSV2
> To: "histonet <@t> lists.utsouthwestern.edu"
>        <histonet <@t> lists.utsouthwestern.edu>
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> I am currently having issues with our HSV1 and HSV2 staining tissue components that it shouldn't be/background.  We are running Ventana Benchmark XTs and Ultras.  Using Dako's polyclonal rabbit concentrate.  Does anyone out there in histoland have a concentration with a protocol that works and looks clean?
>
> Thank you for your help!
>
> Andria B Evans HTL(ASCP)CM
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> End of Histonet Digest, Vol 97, Issue 28
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