[Histonet] Re: Histonet Digest, Vol 97, Issue 25

Thu Dec 22 14:00:35 CST 2011

I would not use any freezing aerosols if there is any suspicion of infection in tissue or cryochamber has been used for prior sectioning without being decontaminated. Depending on how fatty the tissue is you need to be in the region -28 to -35 degs C on the specimen, knife and anti-roll plate, for that you need a decent cryostat.

Best regards

Alan Bright 
Sent from my BlackBerry® wireless device

-----Original Message-----
From: Miha Tesar <sauconym <@t> yahoo.com>
Sender: histonet-bounces <@t> lists.utsouthwestern.edu
Date: Wed, 21 Dec 2011 23:07:26 
To: naveeda arshad<naveedafahim <@t> yahoo.ca>; histonet <@t> lists.utsouthwestern.edu<histonet <@t> lists.utsouthwestern.edu>
Reply-To: Miha Tesar <sauconym <@t> yahoo.com>
Subject: Re: [Histonet] Re: Histonet Digest, Vol 97, Issue 25

Haloha!
Does any one has any idea about how to cut the fat tissue in cryostat (frozen section). Temperature of the chambre/specimen and the thickness of the slice. I use the cryo spray but does not help me much! 
THX Miha and Mary C. 2 all

________________________________
From: naveeda arshad <naveedafahim <@t> yahoo.ca>
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Thursday, December 22, 2011 4:19 AM
Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 25

Hi Does  make in house gram  Positive and negative control in your lab. What kind of tissue is suitable and and i need detail procedure for that.Thanks

--- On Wed, 12/21/11, histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu> wrote:

From: histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu>
Subject: Histonet Digest, Vol 97, Issue 25
To: histonet <@t> lists.utsouthwestern.edu
Received: Wednesday, December 21, 2011, 10:17 PM

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Today's Topics:

   1. RE: Control Slides (Rene J Buesa)
   2. Great Job Opportunity in San Fran Bay Area! (Heidi Hawthorne)

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Message: 1
Date: Wed, 21 Dec 2011 09:36:27 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: RE: [Histonet] Control Slides
To: histonet <@t> lists.utsouthwestern.edu,    MargaretSherwood
    <MSHERWOOD <@t> PARTNERS.ORG>
Message-ID:
    <1324488987.20130.YahooMailClassic <@t> web65716.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Check with a colleague working in a hospital and ask for fresh tissue (for IHC) or (+) pathological cases.
René J.

--- On Wed, 12/21/11, Sherwood, Margaret <MSHERWOOD <@t> PARTNERS.ORG> wrote:

From: Sherwood, Margaret <MSHERWOOD <@t> PARTNERS.ORG>
Subject: RE: [Histonet] Control Slides
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>, histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, December 21, 2011, 12:15 PM

That is ideal Rene, but we are a research lab and don't run the same tissue all the time.

Peggy Sherwood 
Lab Associate, Photopathology 
Wellman Center for Photomedicine (EDR 214) 
Massachusetts General Hospital 
50 Blossom Street 
Boston, MA 02114-2696 
617-724-4839 (voice mail) 
617-726-6983 (lab) 
617-726-1206 (fax) 
msherwood <@t> partners.org 

From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com] 
Sent: Wednesday, December 21, 2011 12:05 PM
To: histonet <@t> lists.utsouthwestern.edu; Sherwood, Margaret
Subject: Re: [Histonet] Control Slides

I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well.
René J. 

--- On Wed, 12/21/11, Sherwood, Margaret <MSHERWOOD <@t> PARTNERS.ORG> wrote:

From: Sherwood, Margaret <MSHERWOOD <@t> PARTNERS.ORG>
Subject: Re: [Histonet] Control Slides
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, December 21, 2011, 11:52 AM

To all:

I know this has come up before, but where do most people buy their (+) control
slides for special stains?  

Thanks!
Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org

The information in this e-mail is intended only for the person to whom it is
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Message: 2
Date: Wed, 21 Dec 2011 09:41:24 -0800
From: Heidi Hawthorne <Heidi.Hawthorne <@t> onassignment.com>
Subject: [Histonet] Great Job Opportunity in San Fran Bay Area!
To: "histonet <@t> lists.utsouthwestern.edu"
    <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <26C4A3B38503BC4CBBF4D986C3BC9B832290D1F5C6 <@t> oasslcexm01.oaifield.onasgn.com>

Content-Type: text/plain; charset="us-ascii"

Hello,

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Sr. Account Executive
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t: (510) 663-8622
c: (510) 435-7326
f: (866) 741-0805
Heidi.Hawthorne <@t> onassignment.com<mailto:Heidi.Hawthorne <@t> onassignment.com>
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NASDAQ: ASGN

People First.

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