[Histonet] proximity of proteins for co-localization via IF staining

[Kim Merriam]

Hi Everyone,
 
Does anyone know how close in proximity 2 proteins need to be for them to show up as co-localized via immunofluorescence staining?
 
I am trying to compare routine 2-color IF vs. the Proximity Ligation Assay (which will not show co-localization unless the proteins are withing 40 nM of each other).
 
Thanks,
Kim

Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA