[Histonet] bacteria staining

[Tony Henwood (SCHN)]

Mesru,

Use 1% aqueous Giemsa, stain dehydrated sections 5-10 minutes, dehydrate rapidly (either acetone, air dry or rapid alcohol dehydation), clear and mount

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of mesruh turkekul
Sent: Tuesday, 20 December 2011 9:52 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] bacteria staining

I would like to stain FFPE mouse intestine sections to demonstrate most of the bacteria in the normal flora of the intestine.
Any suggestions for the simplest histological stain that will show most of the bacteria?

Thanks,
Happy holidays!

Mesru
mskcc.org
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