[Histonet] Stain recipe

[Bryan Llewellyn]

The method is found in Culling, Ed. 2., p.251.

Solutions
	1) Alcian blue
	   Alcian blue 8G 1% aqueous  50 mL
	   Acetic acid 1% aqueous     50 mL
	   Filter, and add 10-20 mg thymol.

	2) Phosphomolybdic acid, 1% aqueous
	3) Chlorantine fast red 5B, 0.5% aqueous

Method
1)  Sections to water.
2)  Ehrlich's hematoxylin 10-15 mins.
3)  Tap water until blue.
4)  Alcian blue solution (1), 10 minutes.
5)  Distilled water rinse, a few seconds.
6)  Phosphomolybdic acid (2), 10 minutes.
7)  Distilled water rinse.
8)  Chlorantine fast red (3), 10-15 minutes.
9)  Distilled water rinse.
10) Dehydrate, clear and mount in a resinous medium.

Expected results:
Mucin, mast cell granules, cartilage - blue
Nuclei - Purple-blue
Collagen and ossein - Cherry-red
Cytoplasm and muscle - pale yellow


Bryan Llewellyn



Ross, Catherine wrote:
> All,
>
> Greetings from across the pond!
>
> One of our histology technicians is looking for a recipe for an alcian
> blue/sirius red stain, otherwise known as "Alcian blue 8G with chlorantine
> fast red 5B". It is not one I have used but I suggested tapping into the
> wealth of knowledge on this forum - can anyone help, please?
>
> Thank you in advance,
> Catherine
>
>
> Dr. Catherine L. Ross  DVM MSc(VetPath) MRCVS
> Covance Laboratories (UK) Ltd.
> Otley Road,
> Harrogate, N. Yorks.
> HG3 1PY
>
> T: +44 (0) 1423 848759
> E: catherine.ross <@t> covance.com<mailto:catherine.ross <@t> covance.com>
>
>
>
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