[Histonet] gelatine, silane, super frost plus slides ???

[Lee & Peggy Wenk]

I'll take a stab at it.

All the subbed slides (short for submerged in a solution) have a coating 
that makes them for positively charged. The lab can make a solution to 
submerge the slides, or the vendor can submerge the slides and sell the 
slides pre-made. Or, the material can be placed in the flotation bath, so 
that the charged material is placed on the slide at the time of sectioning.

The purpose is to place more positive charges on the slide, so that the 
proteins in the tissue have something to bind to, more strongly, so the 
tissue won't fall off the slide.

Poly-L-lysine = is a solution with lots of left-handed lysine amino acids, 
which have lots of amines = NH3+

Silane = solution with silicon and NH3+

Gelatin = solution of proteins, made up of amino acids, some of which are 
amines = NH3+

Chrome-Gelatin = solution of proteins (amine NH3+) and chromium potassium 
sulfate, where the chromium is positively charged.

Plus slides = generic term. Could be coated with any of the above, to make 
them more positively charged (+ = plus sign). Need to read the 
manufacturer's information as to which submersion solution they used.

Super frost = I believe refers to the frosted end being coated with a 
thicker material, so that it's easier to write and read the patient's 
information written on the end of the slide.

Which one used = what works best in your lab, at the price you are willing 
to pay. In other words, moslyt personal preference.

P.S. FYI for your student: Older formulations used:
- Elmer's glue = milk casein proteins originally, which are +. Now synthetic 
compound polyvinyl acetate
- Egg albumin = amino acids, with charges
- Blood serum = with albumin = amino acids = charges (hopefully practice 
ended when some blood serum tested positive with HIV)

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

The opinions expressed to not reflect upon Beaumont Hospital

-----Original Message----- 
From: Daniela Bodemer
Sent: Tuesday, December 13, 2011 12:29 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] gelatine, silane, super frost plus slides ???

Hi all,



I am putting information together for students and thought you might be
able to help me.

Gelatine, silane, super frost plus slides. What are the differences and
characteristics and which slide to use for what?



Thank you,



Daniela Bodemer


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