[Histonet] RE: Histonet Digest, Vol 97, Issue 9
Foshey, Annette
AFoshey <@t> chw.org
Wed Dec 7 15:57:04 CST 2011
Histonetters:
I'm looking for a pediatric institution that charges for autopsy services. What is the charge for a routine pediatric autopsy? What is your charge for a complex autopsy?
Thanks in advance for your responses,
Annette Foshey, HT
Histology Department
Children's Hospital of Wisconsin
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: Wednesday, December 07, 2011 11:43 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 97, Issue 9
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Today's Topics:
1. Re: Trying to get in touch with Gayle Callis. (Victor Tobias)
2. SOX11 (Elizabeth.Dickert <@t> hcahealthcare.com)
3. Ventana NexES Special Stainer (Knutson, Deanne)
4. seeking slide handles for racks (Diana Martinez-Longoria)
5. Histo Aide Duties (Matthew Lunetta)
6. Non-Certified Histo Aide (Matthew Lunetta)
7. Re: Non-Certified Histo Aide (Kim Donadio)
8. Lillie, 3rd Edition (Breeden, Sara)
9. elastic fiber stain in dermis of skin (Kalleberg, Kristopher)
10. Re: Histo Aide Duties (Rene J Buesa)
11. Re: elastic fiber stain in dermis of skin (Rene J Buesa)
12. IL-5 (angela smith)
13. adding eosin to tissue (Carol Bryant)
14. RE: Histo Aide Duties (Nails, Felton)
15. Re: Histo Aide Duties (SHANE NELSON)
16. Re: adding eosin to tissue (V. Neubert)
17. Re: Histo Aide Duties (Rene J Buesa)
18. Re: Lillie, 3rd Edition (Geoff McAuliffe)
19. RE: Histo Aide Duties (Mandy O'Connor)
20. Coverslipper (Marcia Funk)
21. RE: Looking for a very good refurbished rotary microtome to
purchase. (Ken Piech)
22. RE: Lillie, 3rd Edition (WILLIAM DESALVO)
23. RE: Coverslipper (Rathborne, Toni)
----------------------------------------------------------------------
Message: 1
Date: Tue, 06 Dec 2011 10:04:14 -0800
From: Victor Tobias <victor <@t> pathology.washington.edu>
Subject: Re: [Histonet] Trying to get in touch with Gayle Callis.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4EDE591E.3040400 <@t> pathology.washington.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
gayle.callis <@t> bresnan.net
Victor Tobias HT(ASCP)
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor <@t> pathology.washington.edu
206-744-2735
206-744-8240 Fax
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On 12/6/2011 9:59 AM, Clough, Bret wrote:
> I would like to get in touch with Gayle Callis but she is no longer at Montana State University. Does anyone know how to reach her and if so, would you be willing to share that information with me or have her contact me?
>
> Thanks,
> Bret Clough
> Texas A&M Health Science Center
> Temple, TX.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 2
Date: Tue, 6 Dec 2011 12:10:27 -0600
From: <Elizabeth.Dickert <@t> hcahealthcare.com>
Subject: [Histonet] SOX11
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0226177269DFDE48A23F40D00634258B15F41D1DD8 <@t> NADCWPMSGCMS10.hca.corpad.net>
Content-Type: text/plain; charset="us-ascii"
Having problem getting abcam sox11 to work, tried abcams recommended dilutions and several more with no luck!
I need the secret!!
E. Ann Dickert
Southern Pathology
2333 McCallie Ave.
Chattanooga, TN. 37404
phone 423.493.6904
fax 432.698.7858
------------------------------
Message: 3
Date: Tue, 6 Dec 2011 14:30:14 -0600
From: "Knutson, Deanne" <DKnutson <@t> primecare.org>
Subject: [Histonet] Ventana NexES Special Stainer
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1E0E2B14C709174B8AC2BE0AE7F76833A2C49C8FE1 <@t> EXCHANGE2K7.staprimecare.org>
Content-Type: text/plain; charset="us-ascii"
We are moving our special stains bench from manual to automation with the Ventana NexES Special Stainer.
I would be interested to hear from other Ventana users. How do you run your controls? Do you stain a control with each stain batch? Or one a day? I can't find any CAP standards on this.
Another question I would like to ask other Ventana users - have you experienced any "down time" with your special stainer? Do you keep reagents handy to run manually when you are down?
Thank you ahead of time for sharing your experiences with me!
Deanne Knutson
Anatomic Pathology Supervisor
St. Alexius Medical Center
701-530-6730
dknutson <@t> primecare.org<mailto:dknutson <@t> primecare.org>
________________________________
This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message.
------------------------------
Message: 4
Date: Tue, 6 Dec 2011 21:11:01 +0000
From: Diana Martinez-Longoria <dmlongoria <@t> ecrmc.org>
Subject: [Histonet] seeking slide handles for racks
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C6E0DF42-88D6-41E3-A18A-38E73A2E7D43 <@t> mimectl>
Content-Type: text/plain; charset="iso-8859-1"
Hello all,
We have desperately seeking handles for our racks that are from Sakura Tissue Tek Slide Baskets 4768. My supervisor has gone to the Sakura website and has not any information about them. We only need the handles since we already have about 30 baskets. Please help. Thanking you guys in advance!
Diana Martinez-Longoria
Histotechnician (ASCP)cm
El Centro Regional Medical Center
phone: 760-339-7267
fax: 760-482-5365
email:dmlongoria <@t> ecrmc.org
ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.
??
------------------------------
Message: 5
Date: Wed, 07 Dec 2011 06:01:31 -0700
From: "Matthew Lunetta" <MLunetta <@t> luhcares.org>
Subject: [Histonet] Histo Aide Duties
To: <histonet <@t> lists.utsouthwestern.edu>,
<histonet-request <@t> lists.utsouthwestern.edu>
Message-ID: <4EDF013B020000A80006D511 <@t> ns.luhcares.org>
Content-Type: text/plain; charset="us-ascii"
Hey Histo Netters,
For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility?
Other than accessioning.
Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical?
I am not fluent in CAP and would like to know what you all think.
Thanks,
Matt Lunetta
BS HT(ASCP)
------------------------------
Message: 6
Date: Wed, 07 Dec 2011 06:52:33 -0700
From: "Matthew Lunetta" <MLunetta <@t> luhcares.org>
Subject: [Histonet] Non-Certified Histo Aide
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4EDF0D31020000A80006D526 <@t> ns.luhcares.org>
Content-Type: text/plain; charset="us-ascii"
Hey Histo Netters,
For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility?
Other than accessioning.
Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical?
I am not fluent in CAP and would like to know what you all think.
Thanks,
Matt Lunetta
BS HT(ASCP)
------------------------------
Message: 7
Date: Wed, 7 Dec 2011 07:25:37 -0800 (PST)
From: Kim Donadio <one_angel_secret <@t> yahoo.com>
Subject: Re: [Histonet] Non-Certified Histo Aide
To: Matthew Lunetta <MLunetta <@t> luhcares.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1323271537.56006.YahooMailNeo <@t> web112305.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
You're probaly going to get a variety response to this depending on the state the person is in. Remember that CAP is an acreditation organization made up of peers that help each other stay within the guidlines of other state or federal regulatory agencies, such as CLIA and AHCA. < My version of the definition.
?
This question was ask in the march addition of CAP. Link here: http://www.cap.org/apps/docs/education/lapaudio/pdf/031710_qa.pdf?
?
CAP accreditation = CMS $ from my understanding as well. http://wwwn.cdc.gov/clia/docs/fr12se01n.htm? Good article about this relationship
?
For states such as the one I am in. Florida, only florida licensed histotechs can cut, embed, special stains, etc. to my knowledge in a hospital setting.I beleive there is an ecception for private dermatology labs performing MOHS. My understanding is that IHC and grossing are still high complex and is held to more stringent regulations. Someone correct me if i am wrong please.
?
So I would see if your staff meet CLIA guidelines for moderatly complex testing and then look at your states requirements.
?
Heres the guidelines on standard technical help. http://wwwn.cdc.gov/clia/docs/fr12se01n.htm
?
The CLIA web site is so much fun to navigate, so I hope this answers some of your questions or at least gives you some reference points.
?
Kim Donadio
?
?
?
________________________________
From: Matthew Lunetta <MLunetta <@t> luhcares.org>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, December 7, 2011 8:52 AM
Subject: [Histonet] Non-Certified Histo Aide
Hey Histo Netters,
For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility?
Other than accessioning.
Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical?
I am not fluent in CAP and would like to know what you all think.
Thanks,
Matt Lunetta
BS HT(ASCP)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Wed, 7 Dec 2011 08:27:00 -0700
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Lillie, 3rd Edition
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<02C099024072804EA34F5906BAC30A41062C17 <@t> nmdamailsvr.nmda.ad.nmsu.edu>
Content-Type: text/plain; charset="us-ascii"
In honor of my impending RETIREMENT on April 1, 2012, I have decided to
offer for sale my copy of Histopathologic Technic and Practical
Histochemistry by R.D. Lillie, 3rd edition (1964). It is complete and
in very good condition; pages unbent, minor page darkening (age) and
comes with a FREE autograph in my maiden name. First offer of eighty
dollars ($80.00) takes it and I'll pay the postage to get it to you.
I've got to pay for my bon-bons and TV cable bill somehow!
First offer gets it.
Sally Breeden, HT(ASCP)
New Mexico Department of Agriculture
Veterinary Diagnostic Services
1101 Camino de Salud NE
Albuquerque, NM 87102
505-383-9278 (Histology Lab)
------------------------------
Message: 9
Date: Wed, 7 Dec 2011 10:54:32 -0500
From: "Kalleberg, Kristopher" <Kristopher.Kalleberg <@t> unilever.com>
Subject: [Histonet] elastic fiber stain in dermis of skin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0E6BC087F70F9C47ACFF2C203D6E329C0CA6D747 <@t> NTRSEVS30002.s3.ms.unilever.com>
Content-Type: text/plain; charset="US-ASCII"
I am currently running a study to determine the increase in elastin in
the dermis of skin due to certain treatments applied to skin. These
samples will be FFPE after the study concludes. Does anyone have
suggestions on which type of elastin stain works best for FFPE skin
samples? Are there any commercially available stains that work well and
are ready to use? Any help would be greatly appreciated. Thank you in
advance.
Kris
------------------------------
Message: 10
Date: Wed, 7 Dec 2011 07:54:55 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Histo Aide Duties
To: histonet <@t> lists.utsouthwestern.edu,
histonet-request <@t> lists.utsouthwestern.edu, Matthew Lunetta
<MLunetta <@t> luhcares.org>
Message-ID:
<1323273295.32252.YahooMailClassic <@t> web65716.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Embedding NO
Cutting ABSOLUTELY NO
Staining, ONLY if with auto-stainer (putting slides in/out of the instrument)
Cover-slipping ONLY with automatic instrument (slides in/out)
Sticking labels to hand written slides if the slides are not pre-written
Filing slides and blocks YES
Matching slides with paper work YES
Delivering slides to pathologists YES
Arranging blocks/slides to cut YES
Anything that does not involve a technical skill, training or certification.
A good laboratory aid doing all the above mentioned tasks can improve the histotechnologists productivity 2.5 times
Ren? J.
--- On Wed, 12/7/11, Matthew Lunetta <MLunetta <@t> luhcares.org> wrote:
From: Matthew Lunetta <MLunetta <@t> luhcares.org>
Subject: [Histonet] Histo Aide Duties
To: histonet <@t> lists.utsouthwestern.edu, histonet-request <@t> lists.utsouthwestern.edu
Date: Wednesday, December 7, 2011, 8:01 AM
Hey Histo Netters,
For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility?
Other than accessioning.
Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical?
I am not fluent in CAP and would like to know what you all think.
Thanks,
Matt Lunetta
BS HT(ASCP)
-----Inline Attachment Follows-----
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Wed, 7 Dec 2011 08:00:19 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] elastic fiber stain in dermis of skin
To: histonet <@t> lists.utsouthwestern.edu, KristopherKalleberg
<Kristopher.Kalleberg <@t> unilever.com>
Message-ID:
<1323273619.94583.YahooMailClassic <@t> web65714.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Use the van Gieson stain. Just make sure that ALL the reagents are fresh (specially the iron hematoxylin). Try ARTERY as a (+) control.
Ren? J.
--- On Wed, 12/7/11, Kalleberg, Kristopher <Kristopher.Kalleberg <@t> unilever.com> wrote:
From: Kalleberg, Kristopher <Kristopher.Kalleberg <@t> unilever.com>
Subject: [Histonet] elastic fiber stain in dermis of skin
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, December 7, 2011, 10:54 AM
I am currently running a study to determine the increase in elastin in
the dermis of skin due to certain treatments applied to skin.? These
samples will be FFPE after the study concludes.? Does anyone have
suggestions on which type of elastin stain works best for FFPE skin
samples?? Are there any commercially available stains that work well and
are ready to use?? Any help would be greatly appreciated.? Thank you in
advance.
Kris
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Wed, 7 Dec 2011 08:16:05 -0800 (PST)
From: angela smith <we3smitty <@t> yahoo.com>
Subject: [Histonet] IL-5
To: Histonet Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1323274565.63625.YahooMailClassic <@t> web125406.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Does anyone know of a contract lab or facility that is performing IL-5 IHC on paraffin embedded tissue?? I have contacted US Labs, Labcorp and Genzyme and they do not.
Thank you
Angela Smith
Histology Supervisor
Children's Mercy Hospital
?
------------------------------
Message: 13
Date: Wed, 7 Dec 2011 11:17:50 -0500
From: Carol Bryant <cbrya <@t> lexclin.com>
Subject: [Histonet] adding eosin to tissue
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC376B3 <@t> EXCHANGESB>
Content-Type: text/plain; charset="us-ascii"
What are the pros and cons of adding eosin to tissue as it is processed to increase visibility when embedding with small specimens?
Thank you in advance for your input.
Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com
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Message: 14
Date: Wed, 7 Dec 2011 10:25:31 -0600
From: "Nails, Felton" <flnails <@t> texaschildrens.org>
Subject: RE: [Histonet] Histo Aide Duties
To: 'Rene J Buesa' <rjbuesa <@t> yahoo.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>,
"histonet-request <@t> lists.utsouthwestern.edu"
<histonet-request <@t> lists.utsouthwestern.edu>, Matthew Lunetta
<MLunetta <@t> luhcares.org>
Message-ID:
<C1FE5960057C084CA389CE977790629001829E929E <@t> TCDMSG01.ad.TexasChildrensHospital.org>
Content-Type: text/plain; charset="iso-8859-1"
This sounds like union rules not CAP rules
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, December 07, 2011 9:55 AM
To: histonet <@t> lists.utsouthwestern.edu; histonet-request <@t> lists.utsouthwestern.edu; Matthew Lunetta
Subject: Re: [Histonet] Histo Aide Duties
Embedding NO
Cutting ABSOLUTELY NO
Staining, ONLY if with auto-stainer (putting slides in/out of the instrument) Cover-slipping ONLY with automatic instrument (slides in/out) Sticking labels to hand written slides if the slides are not pre-written Filing slides and blocks YES Matching slides with paper work YES Delivering slides to pathologists YES Arranging blocks/slides to cut YES Anything that does not involve a technical skill, training or certification.
A good laboratory aid doing all the above mentioned tasks can improve the histotechnologists productivity 2.5 times Ren? J.
--- On Wed, 12/7/11, Matthew Lunetta <MLunetta <@t> luhcares.org> wrote:
From: Matthew Lunetta <MLunetta <@t> luhcares.org>
Subject: [Histonet] Histo Aide Duties
To: histonet <@t> lists.utsouthwestern.edu, histonet-request <@t> lists.utsouthwestern.edu
Date: Wednesday, December 7, 2011, 8:01 AM
Hey Histo Netters,
For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility?
Other than accessioning.
Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical?
I am not fluent in CAP and would like to know what you all think.
Thanks,
Matt Lunetta
BS HT(ASCP)
-----Inline Attachment Follows-----
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Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 15
Date: Wed, 7 Dec 2011 08:25:33 -0800 (PST)
From: SHANE NELSON <nelsonrnch <@t> verizon.net>
Subject: Re: [Histonet] Histo Aide Duties
To: Histonet <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1323275133.35545.YahooMailNeo <@t> web84516.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Rene could you please explain why coverslipping is a technical skill. It would seem coverslipping slides would be like labeling slides with just little bit more skill. Thank you in advance for your thoughts.
?
HAPPY HOLIDAYS,
?
PATTI RUBEN-NELSON? H.T.(ASCP)
SUPERVISOR/DGC
P.O. BOX 412
CABAZON, CA. 92230
cell (909) 841-9761
nelsonrnch <@t> verizon.net
?
?
CONFIDENTIALITY NOTICE:
This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants?
?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable
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such information is strictly prohibited and may be unlawful.? If you are not
the addressee, please promptly delete this message and notify the sender of
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________________________________
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
To: histonet <@t> lists.utsouthwestern.edu; histonet-request <@t> lists.utsouthwestern.edu; Matthew Lunetta <MLunetta <@t> luhcares.org>
Sent: Wednesday, December 7, 2011 7:54 AM
Subject: Re: [Histonet] Histo Aide Duties
Embedding NO
Cutting ABSOLUTELY NO
Staining, ONLY if with auto-stainer (putting slides in/out of the instrument)
Cover-slipping ONLY with automatic instrument (slides in/out)
Sticking labels to hand written slides if the slides are not pre-written
Filing slides and blocks YES
Matching slides with paper work YES
Delivering slides to pathologists YES
Arranging blocks/slides to cut YES
Anything that does not involve a technical skill, training or certification.
A good laboratory aid doing all the above mentioned tasks can improve the histotechnologists productivity 2.5 times
Ren? J.
--- On Wed, 12/7/11, Matthew Lunetta <MLunetta <@t> luhcares.org> wrote:
From: Matthew Lunetta <MLunetta <@t> luhcares.org>
Subject: [Histonet] Histo Aide Duties
To: histonet <@t> lists.utsouthwestern.edu, histonet-request <@t> lists.utsouthwestern.edu
Date: Wednesday, December 7, 2011, 8:01 AM
Hey Histo Netters,
For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility?
Other than accessioning.
Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical?
I am not fluent in CAP and would like to know what you all think.
Thanks,
Matt Lunetta
BS HT(ASCP)
-----Inline Attachment Follows-----
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 16
Date: Wed, 07 Dec 2011 17:26:29 +0100
From: "V. Neubert" <histonet.nospam <@t> vneubert.com>
Subject: Re: [Histonet] adding eosin to tissue
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4EDF93B5.2050609 <@t> vneubert.com>
Content-Type: text/plain; charset=ISO-8859-1
I cannot see cons right now, but histoland: please feel free to correct me.
Eosin is soluble in water, and as dewaxing most certainly will end in
water, the eosin will be washed out by 70% EtOH and distilled water.
Check your eosin to be sure.
Am 07.12.2011 17:17, schrieb Carol Bryant:
> What are the pros and cons of adding eosin to tissue as it is processed to increase visibility when embedding with small specimens?
> Thank you in advance for your input.
>
> Carol Bryant, CT (ASCP)
> Cytology/Histology Manager
> Lexington Clinic
> Phone (859) 258-4082
> Fax (859) 258-4081
> cbrya <@t> lexclin.com
>
>
>
> NOTICE OF CONFIDENTIALITY
>
> This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege.
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------------------------------
Message: 17
Date: Wed, 7 Dec 2011 09:10:11 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Histo Aide Duties
To: Histonet <Histonet <@t> lists.utsouthwestern.edu>, SHANE NELSON
<nelsonrnch <@t> verizon.net>
Message-ID:
<1323277811.11875.YahooMailClassic <@t> web65706.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Cover-slipping involves several decisions about size of the covers-lip, cleaning excess mounting medium and the like.
Is the final step in the preparation of the slide and could also include a quality control about the staining that could result in returning the slide to re-stain. That type of decision involves looking the section under the microscope and that is absolutely outside the qualification of an aide.
Ren? J.
--- On Wed, 12/7/11, SHANE NELSON <nelsonrnch <@t> verizon.net> wrote:
From: SHANE NELSON <nelsonrnch <@t> verizon.net>
Subject: Re: [Histonet] Histo Aide Duties
To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
Cc: "rjbuesa <@t> yahoo.com" <rjbuesa <@t> yahoo.com>
Date: Wednesday, December 7, 2011, 11:25 AM
Rene could you please explain why coverslipping is a technical skill. It would seem coverslipping slides would be like labeling slides with just little bit more skill. Thank you in advance for your thoughts.
?
HAPPY HOLIDAYS,
?
PATTI RUBEN-NELSON? H.T.(ASCP)
SUPERVISOR/DGC
P.O. BOX 412
CABAZON, CA. 92230
cell (909) 841-9761
nelsonrnch <@t> verizon.net
?
?
CONFIDENTIALITY NOTICE:
This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants?
?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable
law.? Unauthorized forwarding, printing, copying, distribution, or use of
such information is strictly prohibited and may be unlawful.? If you are not
the addressee, please promptly delete this message and notify the sender of
the delivery error by e-mail or you may call? 909-841-9761.
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
To: histonet <@t> lists.utsouthwestern.edu; histonet-request <@t> lists.utsouthwestern.edu; Matthew Lunetta <MLunetta <@t> luhcares.org>
Sent: Wednesday, December 7, 2011 7:54 AM
Subject: Re: [Histonet] Histo Aide Duties
Embedding NO
Cutting ABSOLUTELY NO
Staining, ONLY if with auto-stainer (putting slides in/out of the instrument)
Cover-slipping ONLY with automatic instrument (slides in/out)
Sticking labels to hand written slides if the slides are not pre-written
Filing slides and blocks YES
Matching slides with paper work YES
Delivering slides to pathologists YES
Arranging blocks/slides to cut YES
Anything that does not involve a technical skill, training or certification.
A good laboratory aid doing all the above mentioned tasks can improve the histotechnologists productivity 2.5 times
Ren? J.
--- On Wed, 12/7/11, Matthew Lunetta <MLunetta <@t> luhcares.org> wrote:
From: Matthew Lunetta <MLunetta <@t> luhcares.org>
Subject: [Histonet] Histo Aide Duties
To: histonet <@t> lists.utsouthwestern.edu, histonet-request <@t> lists.utsouthwestern.edu
Date: Wednesday, December 7, 2011, 8:01 AM
Hey Histo Netters,
For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility?
Other than accessioning.
Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical?
I am not fluent in CAP and would like to know what you all think.
Thanks,
Matt Lunetta
BS HT(ASCP)
-----Inline Attachment Follows-----
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 18
Date: Wed, 07 Dec 2011 12:14:51 -0500
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] Lillie, 3rd Edition
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4EDF9F0B.3020801 <@t> umdnj.edu>
Content-Type: text/plain; CHARSET=US-ASCII; format=flowed
I wonder what my 4th edition (1976) is worth?
Geoff
On 12/7/2011 10:27 AM, Breeden, Sara wrote:
> In honor of my impending RETIREMENT on April 1, 2012, I have decided to
> offer for sale my copy of Histopathologic Technic and Practical
> Histochemistry by R.D. Lillie, 3rd edition (1964). It is complete and
> in very good condition; pages unbent, minor page darkening (age) and
> comes with a FREE autograph in my maiden name. First offer of eighty
> dollars ($80.00) takes it and I'll pay the postage to get it to you.
> I've got to pay for my bon-bons and TV cable bill somehow!
>
>
>
> First offer gets it.
>
>
>
> Sally Breeden, HT(ASCP)
>
> New Mexico Department of Agriculture
>
> Veterinary Diagnostic Services
>
> 1101 Camino de Salud NE
>
> Albuquerque, NM 87102
>
> 505-383-9278 (Histology Lab)
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
**********************************************
------------------------------
Message: 19
Date: Wed, 7 Dec 2011 17:26:14 +0000
From: Mandy O'Connor <Amanda <@t> YPII.com>
Subject: RE: [Histonet] Histo Aide Duties
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8287D830EC5C904AA3E482C75773A03B0A0ECBBB <@t> EX1.YPIILab.YPII.com>
Content-Type: text/plain; charset="us-ascii"
Hi guys and gals, I have to comment on this subject. This is where I wish there was a clear cut answer in deciphering what duties an aide vs. HT vs. HTL should and shouldn't do. I work in a private pathology lab where we have a little of everything-an HTL with BS in science, an HT with a 2 yr, an HT with no education, and an aide with only a couple college credits. Everyone is expected to know everything-embedding, cutting, staining, IHC, and also grossing. I highly do not agree with the expectations.
Is there some documentation that actually says what the limits are for unqualified people? Also we need to question if they "should" be doing these duties.
Mandy O'Connor, HTL(ASCP)
Yellowstone Pathology Institute
Billings, MT
amanda <@t> ypii.com<mailto:amanda <@t> ypii.com>
------------------------------
Message: 20
Date: Wed, 07 Dec 2011 12:27:12 -0500
From: "Marcia Funk" <FUNKM <@t> mercyhealth.com>
Subject: [Histonet] Coverslipper
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4EDF4D90020000AC00009FCE <@t> nodcdmg2.no.trinity-health.org>
Content-Type: text/plain; charset=US-ASCII
Hello from the North land- We are having coverslipper issues with our Lecia.
We have noticed that they coverslipes are not
coming in clean as before and dropping off the
red cups. We have changed the red cups but I
have a concern with the dirty and dusty cover
slips. If you have a minute please share what
brand you are using. Thanks Much - Marcia
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-428-7907
------------------------------
Message: 21
Date: Wed, 7 Dec 2011 12:32:44 -0500
From: Ken Piech <kenp <@t> labx.com>
Subject: RE: [Histonet] Looking for a very good refurbished rotary
microtome to purchase.
To: Rene J Buesa <rjbuesa <@t> yahoo.com>, "Histonet list serv."
<histonet <@t> lists.utsouthwestern.edu>, BretClough
<Clough <@t> medicine.tamhsc.edu>
Message-ID: <85e2bd2725a4c7fa54f76815bde7103a <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Check the Microtome category on LabX, we are a marketplace just like eBay
but for lab professionals wanting to purchase new and used lab equipment
mainly. http://www.labx.com/v2/newad.cfm?catID=39
You can post a free Equipment Wanted ad as well.
Best Regards,
Ken Piech
www.labx.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J
Buesa
Sent: December-06-11 11:50 AM
To: Histonet list serv.; BretClough
Subject: Re: [Histonet] Looking for a very good refurbished rotary
microtome to purchase.
Check eBay. Sometimes they have good microtomes listed.
Ren? J.
--- On Tue, 12/6/11, Clough, Bret <Clough <@t> medicine.tamhsc.edu> wrote:
From: Clough, Bret <Clough <@t> medicine.tamhsc.edu>
Subject: [Histonet] Looking for a very good refurbished rotary microtome
to purchase.
To: "Histonet list serv." <histonet <@t> lists.utsouthwestern.edu>
Date: Tuesday, December 6, 2011, 11:19 AM
I am trying to locate a really good inexpensive rotary microtome? to
purchase for our research lab. Any thoughts as to where to look and what
model to get? We currently have a Lieca 1512 and are looking to have a
backup. Any thoughts or suggestions would be greatly appreciated.
Thanks ,
? ? Bret Clough
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 22
Date: Wed, 7 Dec 2011 10:35:47 -0700
From: WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
Subject: RE: [Histonet] Lillie, 3rd Edition
To: <mcauliff <@t> umdnj.edu>, histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY151-W21C68785E28C61F5C6871B91BB0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
I just checked and Amazon has used, good condition, hard cover for $6.00. No matter the cost, the book is priceless if you work in a Histology lab.
William DeSalvo, B.S., HTL(ASCP)
> Date: Wed, 7 Dec 2011 12:14:51 -0500
> From: mcauliff <@t> umdnj.edu
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Lillie, 3rd Edition
>
> I wonder what my 4th edition (1976) is worth?
>
> Geoff
>
> On 12/7/2011 10:27 AM, Breeden, Sara wrote:
> > In honor of my impending RETIREMENT on April 1, 2012, I have decided to
> > offer for sale my copy of Histopathologic Technic and Practical
> > Histochemistry by R.D. Lillie, 3rd edition (1964). It is complete and
> > in very good condition; pages unbent, minor page darkening (age) and
> > comes with a FREE autograph in my maiden name. First offer of eighty
> > dollars ($80.00) takes it and I'll pay the postage to get it to you.
> > I've got to pay for my bon-bons and TV cable bill somehow!
> >
> >
> >
> > First offer gets it.
> >
> >
> >
> > Sally Breeden, HT(ASCP)
> >
> > New Mexico Department of Agriculture
> >
> > Veterinary Diagnostic Services
> >
> > 1101 Camino de Salud NE
> >
> > Albuquerque, NM 87102
> >
> > 505-383-9278 (Histology Lab)
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
>
>
> --
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583
> mcauliff <@t> umdnj.edu
> **********************************************
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 23
Date: Wed, 7 Dec 2011 17:37:21 +0000
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
Subject: RE: [Histonet] Coverslipper
To: "'Marcia Funk'" <FUNKM <@t> mercyhealth.com>,
"Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3AD061FE740D464FAC7BF6B5CFB7570711F64EF2 <@t> SMCMAIL01.somerset-healthcare.com>
Content-Type: text/plain; charset="us-ascii"
StatLab coverglass. It also comes in a 2oz size which is nice for the coverglass dispensers on the CV5030 that we have.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marcia Funk
Sent: Wednesday, December 07, 2011 12:27 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Coverslipper
Hello from the North land- We are having coverslipper issues with our Lecia.
We have noticed that they coverslipes are not
coming in clean as before and dropping off the
red cups. We have changed the red cups but I
have a concern with the dirty and dusty cover
slips. If you have a minute please share what
brand you are using. Thanks Much - Marcia
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-428-7907
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
CONFIDENTIALITY NOTICE
This message and any included attachments are from Somerset Medical Center
and are intended only for the addressee. The information contained in this
message is confidential and may contain privileged, confidential,
proprietary and/or trade secret information entitled to protection and/or
exemption from disclosure under applicable law. Unauthorized forwarding,
printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful. If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
at 908-685-2200, ext. 4050.
Be sure to visit Somerset Medical Center's Web site -
www.somersetmedicalcenter.com - for the most up-to-date news,
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