[Histonet] elastic fiber stain in dermis of skin

Rene J Buesa rjbuesa <@t> yahoo.com
Wed Dec 7 10:00:19 CST 2011


Use the van Gieson stain. Just make sure that ALL the reagents are fresh (specially the iron hematoxylin). Try ARTERY as a (+) control.
René J.

--- On Wed, 12/7/11, Kalleberg, Kristopher <Kristopher.Kalleberg <@t> unilever.com> wrote:


From: Kalleberg, Kristopher <Kristopher.Kalleberg <@t> unilever.com>
Subject: [Histonet] elastic fiber stain in dermis of skin
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, December 7, 2011, 10:54 AM


I am currently running a study to determine the increase in elastin in
the dermis of skin due to certain treatments applied to skin.  These
samples will be FFPE after the study concludes.  Does anyone have
suggestions on which type of elastin stain works best for FFPE skin
samples?  Are there any commercially available stains that work well and
are ready to use?  Any help would be greatly appreciated.  Thank you in
advance.



Kris

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