[Histonet] RE: Histonet Digest, Vol 97, Issue 6
Santiago, Albert
Albert.Santiago <@t> uphs.upenn.edu
Mon Dec 5 12:10:51 CST 2011
Hi Toni, we've had that problem also. The best solution we've been able to find out is a pen called KP Marker Plus. You can find it at www.klinipath.com. I hope that helps, good luck.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Monday, December 05, 2011 1:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 97, Issue 6
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Today's Topics:
1. AW: [Histonet] Re: Clostridium difficile - what do you do
(Gudrun Lang)
2. Re: Re: Clostridium difficile - what do you do (Bob Richmond)
3. nissl after permount? (Teresa Iglesias)
4. Re: nissl after permount? (John Kiernan)
5. c-myc (Angela Bitting)
6. Tissue Loss (Pardue, Judith)
7. Re: Tissue Loss (Rene J Buesa)
8. RE: cassette marker (Goins, Tresa)
9. Re: RE: cassette marker (Nicole Tatum)
10. RE: Tissue Loss (Shirley A. Powell)
11. Cassettes not fitting in molds (Jill Cox)
12. Question - CPT Coding 88321 (Richard Cartun)
----------------------------------------------------------------------
Message: 1
Date: Sun, 4 Dec 2011 19:28:56 +0100
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] Re: Clostridium difficile - what do you do
To: "'Bob Richmond'" <rsrichmond <@t> gmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <6E6CC4C0842740E783D6F4C99D894308 <@t> dielangs.at>
Content-Type: text/plain; charset="us-ascii"
Nom.: Clostridium difficile
Gen.: Clostridii difficilis
Dat.: Clostridio difficili
Akk.: Clostridium difficile
Voc.: Clostridio difficili
I learned this pronounciation: 'klostri:dium di'fi:tsile in school. C is a
"k" (like club) if a consonant follows and a "c" (like ts) if a vocal
follows.
But it's horrible, that after 5 years latin in school, I'm not able to form
a sensefull sentence in this dead language.
Clostridium difficile bacterium horribile est.
Gudrun Lang
Austria
"Tu felix Austria nube!" = you lucky Austria marry; that was the better way
to get more land.
------------------------------
Message: 2
Date: Sun, 4 Dec 2011 15:31:19 -0500
From: Bob Richmond <rsrichmond <@t> gmail.com>
Subject: Re: [Histonet] Re: Clostridium difficile - what do you do
To: gu.lang <@t> gmx.at
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAOKsRH5gRHhTMXOdRWevjAJOZCLA+vs4=MYZPgTSPSDBcmsG9w <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
di'fi:tsile is the German received pronunciation. I use it when I
speak German, just as I use the English received pronunciation when I
speak English.
I didn't know "Tu felix Austria nube!" - When was it said, and in what
historical setting?
Hope you enjoyed the Mozart!
Bob Richmond
Samurai Pathologist
Knoxville, Tennessee
****************************************
On Sun, Dec 4, 2011 at 1:28 PM, Gudrun Lang <gu.lang <@t> gmx.at> wrote:
> Nom.: Clostridium difficile
> Gen.: Clostridii difficilis
> Dat.: Clostridio difficili
> Akk.: Clostridium difficile
> Voc.: Clostridio difficili
>
> I learned this pronounciation: 'klostri:dium di'fi:tsile in school. C is a
> "k" (like club) if a consonant follows and a "c" (like ts) if a vocal
> follows.
>
> But it's horrible, that after 5 years latin in school, I'm not able to form
> a sensefull sentence in this dead language.
> Clostridium difficile bacterium horribile est.
>
> Gudrun Lang
> Austria
> "Tu felix Austria nube!" = you lucky Austria marry; that was the better way
> to get more land.
------------------------------
Message: 3
Date: Sun, 4 Dec 2011 16:55:44 -0600
From: Teresa Iglesias <tliglesias <@t> ucdavis.edu>
Subject: [Histonet] nissl after permount?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAKJrUsBtd=5Aw8Q+_PO90SKLQsRfK2U3ecySDyE17uGpfWzg7Q <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi all,
I'm new to this so this may be a very dumb question but here goes.
If you have already stained for IEGs (Zenk and cFos protein) in brain
tissue and adhered coverslips with permount to the slides is it possible to
then stain with nissl (after removing the covers, of course)?
I'm having a hard time locating certain nuclei now (they were obvious
before staining for IEGs and mounting) so I'm looking for a way to
corroborate that I am looking in the right areas.
As an alternative, I can stain new sections with nissl that have been kept
in cryoprotectant and are free of IEG staining. IF I take this route, would
I have to stain a replicate set of sections for ALL individuals (birds) so
that I compare each IEG stained section to a nissl section that came from
the same bird? In other words, I have 100 slides with 3+ sections per slide
with IEG staining, will I need 100 slides with the same sections stained
for nissl?
Thanks for the help!!
-Teresa
--
______________________________
Teresa Iglesias
Graduate Group in Animal Behavior
Department of Evolution and Ecology
University of California-Davis
------------------------------
Message: 4
Date: Mon, 05 Dec 2011 02:03:21 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] nissl after permount?
To: Teresa Iglesias <tliglesias <@t> ucdavis.edu>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <73c0a67ab443a.4edc2669 <@t> uwo.ca>
Content-Type: text/plain; CHARSET=US-ASCII
The usual approach would be to do Nissl stains on nearby (ideally adjacent) sections to those immunostained for the immediate early gene products. If you have no unstained slides (from bad planning), you will need to remove the coverslips from some of your slides, rehydrate and then do your Nissl stain. If the immunohistochemical product was oxidized DAB (brown), this will remain in place, and your sections will also have nuclear chromatin and nucleolar and cytoplasmic rRNA coloured blue, violet or red according to your choice of Nissl stain.
Removing coverslips, extracting resinous mountant, rehydration and restaining is a tedious and time consuming job (days to a few weeks), especially if the slides are bearing thick sections (50-200um) or whole-mounts.
As a graduate student, you should have an experienced faculty member to advise you. If your boss told you to ask on the Internet, he isn't earning his salary.
There are many experienced histotechnologists at the University of California at Davis. Histotechs love to pass on their experience, learning and practical advice. You should still chase up your academic supervisor, who is paid from your "tuition" fees.
John Kiernan
Anatomy, UWO
London, Canada
= = =
On 04/12/11, Teresa Iglesias <tliglesias <@t> ucdavis.edu> wrote:
>
> Hi all,
> I'm new to this so this may be a very dumb question but here goes.
> If you have already stained for IEGs (Zenk and cFos protein) in brain
> tissue and adhered coverslips with permount to the slides is it possible to
> then stain with nissl (after removing the covers, of course)?
>
> I'm having a hard time locating certain nuclei now (they were obvious
> before staining for IEGs and mounting) so I'm looking for a way to
> corroborate that I am looking in the right areas.
>
> As an alternative, I can stain new sections with nissl that have been kept
> in cryoprotectant and are free of IEG staining. IF I take this route, would
> I have to stain a replicate set of sections for ALL individuals (birds) so
> that I compare each IEG stained section to a nissl section that came from
> the same bird? In other words, I have 100 slides with 3+ sections per slide
> with IEG staining, will I need 100 slides with the same sections stained
> for nissl?
>
> Thanks for the help!!
>
> -Teresa
>
> --
> ______________________________
> Teresa Iglesias
> Graduate Group in Animal Behavior
> Department of Evolution and Ecology
> University of California-Davis
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 5
Date: Mon, 05 Dec 2011 09:15:05 -0500
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
Subject: [Histonet] c-myc
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4EDC8B98.2B7F.00C9.1 <@t> geisinger.edu>
Content-Type: text/plain; charset="us-ascii"
Does anyone send slides out for c-myc staining by IHC? Where do you send to?
Thanks.
Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
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X-GWTYPE:USER
FN:Bitting, Angela
TEL;WORK:570-271-6844
ORG:;Histology
EMAIL;WORK;PREF;NGW:AKBITTING <@t> geisinger.edu
N:Bitting;Angela
END:VCARD
------------------------------
Message: 6
Date: Mon, 5 Dec 2011 07:24:17 -0700
From: "Pardue, Judith" <Judith_Pardue <@t> memorial.org>
Subject: [Histonet] Tissue Loss
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14B823F24E628E49BBFAD704E4BAB89A379B20 <@t> chimsx03.CHI.catholichealth.net>
Content-Type: text/plain; charset="ISO-8859-1"
I would like some input on a situation I am faced with in our lab. We
are constantly having tissue coming off the slide on GI bx's. This is
coming from one tech, she is a senior tech and takes offense to
questioning her about the problem. From what I can tell all of the techs
are following the same protocal.
Judith Gale Pardue HT(ASCP), QIHC
Histology Supervisor
423-495-5756
judith_pardue <@t> memorial.org
Memorial Healthcare System
To the world I'm one, to one I'm the world
This electronic mail and any attached documents is intended solely for the named addressee(s) and contains confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.
------------------------------
Message: 7
Date: Mon, 5 Dec 2011 06:50:31 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Tissue Loss
To: histonet <@t> lists.utsouthwestern.edu, JudithPardue
<Judith_Pardue <@t> memorial.org>
Message-ID:
<1323096631.21102.YahooMailClassic <@t> web65706.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
First of all disregard any "offense" any tech may take when you have something to say about the work result. The patient's care overrules "any offense".
Tissues can come off, specially in GI biopsies if they dry out too much during processing with a general protocol where dehydration results too much for them.
Also cutting too fast and not letting the sections to drain correctly before drying in the oven to be stained, can cause them to fall off.
Check how your "senior tech" cuts, and if it is too fast, tell her to slow down a bit.
Compare how she works and how the other techs work and if you see any technical difference, that is probably the cause and "offended" or not tell her to follow standard procedure.
A tech should care for the quality of the work and in the histology laboratory there is no place for "histo-divas".
René J.
--- On Mon, 12/5/11, Pardue, Judith <Judith_Pardue <@t> memorial.org> wrote:
From: Pardue, Judith <Judith_Pardue <@t> memorial.org>
Subject: [Histonet] Tissue Loss
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, December 5, 2011, 9:24 AM
I would like some input on a situation I am faced with in our lab. We
are constantly having tissue coming off the slide on GI bx's. This is
coming from one tech, she is a senior tech and takes offense to
questioning her about the problem. From what I can tell all of the techs
are following the same protocal.
Judith Gale Pardue HT(ASCP), QIHC
Histology Supervisor
423-495-5756
judith_pardue <@t> memorial.org
Memorial Healthcare System
To the world I'm one, to one I'm the world
This electronic mail and any attached documents is intended solely for the named addressee(s) and contains confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.
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------------------------------
Message: 8
Date: Mon, 5 Dec 2011 15:21:43 +0000
From: "Goins, Tresa" <TGoins <@t> mt.gov>
Subject: [Histonet] RE: cassette marker
To: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CA4DF32ED505D94BB55E95487D8E98411F1A0DBF <@t> DOAISD5205.state.mt.ads>
Content-Type: text/plain; charset="us-ascii"
Laboratory Marking Pens from Thermo Scientific (Richard-Allan) Ref 2000. Best for staying on but sensitive to writing on surfaces that are not absolutely dry.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Saturday, December 03, 2011 11:39 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cassette marker
Can anyone recommend a marker for using on cassettes? We currently use pencil, which sometimes smudges. We've tried a few markers already, but some fade, while others hold up well for processing, but won't when placed in decalcifier.
Vendors are welcome to respond.
Thanks,
Toni
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------------------------------
Message: 9
Date: Mon, 5 Dec 2011 10:44:50 -0500 (EST)
From: "Nicole Tatum" <nicole <@t> dlcjax.com>
Subject: Re: [Histonet] RE: cassette marker
To: "Goins, Tresa" <TGoins <@t> mt.gov>, histonet <@t> lists.utsouthwestern.edu
Message-ID:
<4901.208.62.167.196.1323099890.squirrel <@t> webmail.realpages.com>
Content-Type: text/plain;charset=iso-8859-1
I absolutely love mercedes medical pens called platunium line mer marker.
They are smudge prrof and stay on. Love them. Im sure if you called CHAD
at mercedes he could send you a free sample.
Nicole Tatum
Laboratory Marking Pens from Thermo Scientific (Richard-Allan) Ref 2000.
> Best for staying on but sensitive to writing on surfaces that are not
> absolutely dry.
>
>
>
>
>
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rathborne,
> Toni
> Sent: Saturday, December 03, 2011 11:39 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] cassette marker
>
>
>
> Can anyone recommend a marker for using on cassettes? We currently use
> pencil, which sometimes smudges. We've tried a few markers already, but
> some fade, while others hold up well for processing, but won't when placed
> in decalcifier.
>
> Vendors are welcome to respond.
>
> Thanks,
>
> Toni
>
>
>
>
>
>
>
>
>
> CONFIDENTIALITY NOTICE
>
> This message and any included attachments are from Somerset Medical Center
>
> and are intended only for the addressee. The information contained in
> this
>
> message is confidential and may contain privileged, confidential,
>
> proprietary and/or trade secret information entitled to protection and/or
>
> exemption from disclosure under applicable law. Unauthorized forwarding,
>
> printing, copying, distribution, or use of such information is strictly
>
> prohibited and may be unlawful. If you are not the addressee, please
>
> promptly delete this message and notify the sender of the delivery error
>
> by e-mail or you may call Somerset Medical Center's computer Help Desk
>
> at 908-685-2200, ext. 4050.
>
>
>
> Be sure to visit Somerset Medical Center's Web site -
>
> www.somersetmedicalcenter.com - for the most up-to-date news,
>
> event listings, health information and more.
>
> _______________________________________________
>
> Histonet mailing list
>
> Histonet <@t> lists.utsouthwestern.edu
>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 10
Date: Mon, 5 Dec 2011 11:17:27 -0500
From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
Subject: [Histonet] RE: Tissue Loss
To: "Pardue, Judith" <Judith_Pardue <@t> memorial.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9BF995BC0E47744E9673A41486E24EE24AA1325B9B <@t> MERCERMAIL.MercerU.local>
Content-Type: text/plain; charset="us-ascii"
If she is using hand lotion and handles the slides, that will make the come off. She needs to take all the lotion off before cutting slides.
Shirley
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pardue, Judith
Sent: Monday, December 05, 2011 9:24 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Tissue Loss
I would like some input on a situation I am faced with in our lab. We
are constantly having tissue coming off the slide on GI bx's. This is
coming from one tech, she is a senior tech and takes offense to
questioning her about the problem. From what I can tell all of the techs
are following the same protocal.
Judith Gale Pardue HT(ASCP), QIHC
Histology Supervisor
423-495-5756
judith_pardue <@t> memorial.org
Memorial Healthcare System
To the world I'm one, to one I'm the world
This electronic mail and any attached documents is intended solely for the named addressee(s) and contains confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Mon, 5 Dec 2011 08:27:01 -0800 (PST)
From: Jill Cox <jcox90 <@t> yahoo.com>
Subject: [Histonet] Cassettes not fitting in molds
To: "Histonet <@t> Lists. Edu" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1323102421.70461.YahooMailNeo <@t> web161603.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hi all,
I just purchased new metal molds from a new vendor and my cassettes aren't fitting flat into them. Either the front or back is lifted. I use tissue tek uni-cassettes. I will say the molds I purchased weren't Sakura. Any suggestions? Maybe different cassette? Thanks in advance, Jill
Jill Cox, HT ASCP
------------------------------
Message: 12
Date: Mon, 05 Dec 2011 12:17:59 -0500
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: [Histonet] Question - CPT Coding 88321
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4EDCB677.7400.0077.1 <@t> harthosp.org>
Content-Type: text/plain; charset=US-ASCII
I'm confused (what's new!). I'm being told there is no "technical component" (TC) for 88321 (slide consultation). Is that correct? There has to be a technical component to cover accessioning. Thanks for your help.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
------------------------------
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End of Histonet Digest, Vol 97, Issue 6
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