[Histonet] Clostridium difficile - what do you do

[Rob Geske]

Hi Brett,
 
you might want to take a look at the publication linked to below.  2% glutaraldehyde is indicated in some of the (many) references from the publication and you might consider that option (spiking your NBF with 2% glut) if it will not interfere with any of the procedures you have planned for the tissues.  
 
regards & bona fortuna,
rob
 

Guideline for Disinfection and Sterilization in Healthcare Facilities, 2008 William A. Rutala, Ph.D., M.P.H.1,2, David J. Weber, M.D., M.P.H.1,2, and the Healthcare Infection Control Practices Advisory Committee (HICPAC)3 1Hospital Epidemiology University of North Carolina Health Care System Chapel Hill, NC 27514 2Division of Infectious Diseases University of North Carolina School of Medicine Chapel Hill, NC 27599-7030 1
 
 
http://www.cdc.gov/hicpac/pdf/guidelines/Disinfection_Nov_2008.pdf


--- On Fri, 12/2/11, Connolly, Brett M <brett_connolly <@t> merck.com> wrote:


From: Connolly, Brett M <brett_connolly <@t> merck.com>
Subject: [Histonet] Clostridium difficile - what do you do
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Friday, December 2, 2011, 1:30 PM



Histonetters-

We are about to embark on a project involving C.diff infected GI tissue.

We have processes in place for PPE, decontamination/ disinfection etc... but I am looking for any evidence whether or not formalin fixation or subsequent tissue processing will inactivate the spores.

Also, do you take any special precautions in handling/sectioning the tissue /slides after paraffin embedding.

I am not coming up with much info with my web searches, so I am asking for help from any lab that works with C. diff.

Thanks,
Brett

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803





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