[Histonet] Chameleon tissue lifting off

[Thomas, Nancy]

Scott,
Thank you for your recommendation of the Superfrost ultra plus slides.  I have just contacted the company for a sample box.  I received a few other suggestions too, and will keep that info for possible future use.  I will keep your email address, as well and maybe I will be able to help you sometime.
Thanks to others who replied.

Nancy Thomas



From: Scott Parker [mailto:sparker <@t> vt.edu]
Sent: Friday, December 02, 2011 12:55 PM
To: Thomas, Nancy
Subject: Re: [Histonet] Chameleon tissue lifting off

Hello Nancy,

I work exclusively with squamate uterine and placental tissue so I know the frustration you are experiencing. Because placental tissues that I work with are so thin, separation is always a concern especially when using typical heat mediated antigen-retrieval techniques for immunohistochemistry.

I actually have good success with Thermo Scientific Superfrost ultra plus slides. We have best success with 5 um sections and air drying slides slowly on a slide warmer. We then bake the slides for 30 minutes at 60 C. As usual, carefully sectioning is also the first step in achieving good tissue adherence. With delicate reptile placental tissues, any imperfections on the sectioning side will lead to greater tissue detachment. I hope this helps. We should stay in touch as there are not too many of us that work on reptile tissue in the histo-sphere.

Scott

Scott L. Parker, Ph.D.
Assistant Professor of Biology
Department of Biology
Coastal Carolina University
SC 29528-6054
On Wed, Nov 30, 2011 at 10:00 AM, Thomas, Nancy <nto <@t> stowers.org<mailto:nto <@t> stowers.org>> wrote:
I would like to ask anyone who sections chameleon tissue what type of slides they use.  I was having too much lifting while using the superfrost plus slides.  It looked like slides coated with Haupt's solution were highly recommended, so I tried that.  It is so much better, but still there is some lifting.  If someone is successful with sectioning and staining lizard tissue without lifting, please advise me on type of slides, drying times, or anything that might help.
Thank you so much,

Nancy Thomas
Stowers Institute for Medical Research
Kansas City, MO
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