[Histonet] RE: Using methyl benzoate on a VIP 1000

[Anatoli Gleiberman]

This thing was used as an intermediate step in paraffin processing of some difficult tissues such as whole mouse eye or amphibian eggs between xylene and paraffin. Awful smell, but some people like it - benzyl benzoate smells the same and is used as food preservative. Root beer has a strong smell of methyl (or benzyl) benzoate. Don't use it on VIP - your lab will smell during next several years. In my experience, use of cedar oil between xylene and paraffin (clearing in xylene, overnight incubation in cedar oil, short wash with benzene) works even better than methyl benzoate - and smells much better, for sure. 

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman <@t> cbiolabs.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Houston, Ronald
Sent: Friday, December 02, 2011 3:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Using methyl benzoate on a VIP 1000

not familiar with its use nowadays, but I remember it being combined with celloidin (methyl benzoate celloidin) as part of a double-embedding protocol used for autopsy tissues. Personally I have not heard of it being used alone. 

Can still remember "floating" in the room where we had all our open processors with chloroform, methyl benzoate, alcohols, xylene, celloidin and ether in the atmosphere. Oh happy days - we were still high when we got to the pub after work!

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kevin Seguin
Sent: Friday, December 02, 2011 2:40 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Using methyl benzoate on a VIP 1000

I have a customer who would like to use methyl benzoate on a VIP 1000.  Sakura says that it is not on the list of reagents so it is not recommended.  Can anyone shed some light on using methyl benzoate with a VIP 1000 or other tissue processors?  Will this work?  What are the problems?

Thanks!



Best regards,

Kevin Seguin
GMI
www.gmi-inc.com<http://www.gmi-inc.com>
kseguin <@t> gmi-inc.com<mailto:kseguin <@t> gmi-inc.com>
800-745-2710



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