[Histonet] b-cells stain

[Amos Brooks]

Thanks John,
     Since I was writing the message about Gomori's chrome alum hematoxylin
without the procedure in front of me yesterday (not the best idea, I know)
I didn't have the whole formulation available to me. For Gomori's chrome
alum hematoxylin, in addition to potassium dichromate, there is also
chromium potassium sulfate and sulfuric acid as well. By no means is there
any ferric chloride in it and as I mentioned if one tried to make this with
ferric chloride, I doubt it would work for beta cells since according to
Bancroft in *Theory & Practice of Histotechnology* chrome alum has a strong
affinity for sulfonic acid radicals which are produced in the oxidation of
the glycoproteins in the beta cells in earlier steps of the stain.
     So long story short: ferric chloride probably would not work for
Gomori's stain. Sorry if it sounded like I suggested it would. I really
appreciate having folks like John Kiernan around to point out possible
mis-communications like that.

Have a great weekend everyone,
Amos

On Fri, Dec 2, 2011 at 2:04 AM, John Kiernan <jkiernan <@t> uwo.ca> wrote:

> Dear Amos,
> You may have confused some histonetters if any of them try to make up
> Gomori's chrome alum hematoxylin with either potassium dichromate or ferric
> chloride!
> Cheers,
> John Kiernan
> Anatomy, UWO
> London, Canada
> = = =
> On 01/12/11, *Amos Brooks *<amosbrooks <@t> gmail.com> wrote:
>
> Hi,
>    I like to prepare solutions myself as well. As you say though
> paraldehyde is classified as a drug so there are hoops to jump through to
> be able to purchase it. This is one of those times that it totally makes
> sense to let someone else make it up. EMS sells aldehyde fuchsin so it
> saves me the hassle of dealing with the DEA. Better yet the price isn't
> terrible and works great. Here's the info for it:
> RT 26096 Aldehyde Fuchsin Solution 250 ml 24.00
>
> http://www.emsdiasum.com/microscopy/products/histology/staining_solutions.aspx#26091
>     Now as for Gomori's chrome alum hematoxylin, you really are better off
> using the chrome (potassium dichromate if I recall correctly) than ferric
> chloride (Weigerts formulation right?). I don't think the iron hematoxylin
> would be as specific to the beta cells, but hey, if you decide to try it,
> please let me know if it works out for you. Ferric chloride is better to
> work with than potassium dichromate! Heck you could write up a new
> procedure and name it after yourself.
>
> Amos
>
>
> On Thu, Dec 1, 2011 at 1:00 PM,
> <histonet-request <@t> lists.utsouthwestern.edu>wrote:
>
> > Message: 3
> > Date: Thu, 1 Dec 2011 06:25:00 -0800 (PST)
> > From: Silvina Molinuevo <silvinamolinuevo <@t> yahoo.com.ar>
> > Subject: [Histonet] b-cells stain
> > To: "histonet <@t> lists.utsouthwestern.edu"
> >        <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >        <1322749500.19574.YahooMailNeo <@t> web113609.mail.gq1.yahoo.com>
> > Content-Type: text/plain; charset=iso-8859-1
> >
> > Hi Histonetters!!
> > I want to do Chrome Hematoxylin-Phloxin (from Gomori) to show b-cells.
> > Does anybody know if the stain go well with ferrum hematoxylin instead of
> > chrome hematoxylin?
> > I know that the best stain is Aldehyde-fuchsin but I can't import
> > paraldehyde because of legal restrictions about that product in my
> country.
> > Thanks in advance.
> >
> > Dr María Silvina Molinuevo
> > Grupo de Investigacion en
> > Osteopatias y Metabolismo Mineral
> > Departamento de Ciencias Biologicas
> > Facultad de Ciencias Exactas
> > Universidad Nacional de La Plata
> > 47 y 115
> > (1900)La Plata
> > Argentina
> >
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