[Histonet] RE: Histonet slide
Sellestine Ratliff
sratliff <@t> brownpathology.com
Tue Aug 30 14:36:55 CDT 2011
Hi Sheila,
We ran into the problem of tissue not sticking to the slide a few years ago. Our problem was in the water line attached to our stainer. We found out that the city had cut off the water to do some repairs. We disconnected our water and flushed out the stainer line with a 10% bleach solution removing debris
that had changed the pH in the water. Our problem went away. Maybe this is something you can try.
S.Ratliff HT (ACSP)
Brown & Associates
Houston, Texas
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, August 30, 2011 10:42 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 93, Issue 41
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Today's Topics:
1. Histotech position (Bilger, Andrea)
2. Thanks! (Mary Helie)
3. Cost of an H&E (Carol Fields)
4. Re: iliac artery attachment to slides? (Johnson, Teri)
5. Re: Cost of an H&E (Rene J Buesa)
6. training techs (Patsy Ruegg)
7. Charcoal filters for VIP1000 K series (gayle callis)
8. Re: training techs (histotech <@t> imagesbyhopper.com)
9. Re: peggy wenk comments on HT/HTL practical
(histotech <@t> imagesbyhopper.com)
10. RE: Going for the HTL (Jeff and Wanda Gray)
11. Re: peggy wenk comments on HT/HTL practical (Bob Richmond)
12. ACD RNAscope (Louise Renton)
13. supply inventory software (Jean Brinker)
14. Slides (Sheila Haas)
15. Looking for sponsors (Pathology Lab)
16. brazilin (Vanessa Orsini)
17. Capillary Gap Slides (Rob Geske)
18. RE: Re: peggy wenk comments on HT/HTL practical
(Shirley A. Powell)
19. RE: Slides (Shirley A. Powell)
20. Urgent - testing for cobalt in thyroid (Weems, Joyce)
21. RE: peggy wenk comments on HT/HTL practical (sdysart <@t> mirnarx.com)
22. RE: Slides (Smith, Allen)
23. RE: peggy wenk comments on HT/HTL practical (Heath, Nancy L.)
----------------------------------------------------------------------
Message: 1
Date: Mon, 29 Aug 2011 13:16:02 -0400
From: "Bilger, Andrea" <abilger <@t> wellspan.org>
Subject: [Histonet] Histotech position
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6D7752544B308D44A902C0BD0EC7BF5C8721E666 <@t> EXCH02.wellspan.org>
Content-Type: text/plain; charset="US-ASCII"
Histonet members,
York Hospital in York Pennsylvania is looking for a histotech to work day shift Monday through Friday with an occasional 5 hours on Saturday. If you are interested, apply at www.wellspan.org<http://www.wellspan.org> or contact:
Andrea Bilger
Team Leader, Histology
York Hospital
1001 South George St.
York, Pa. 17405
717-851-5040
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------------------------------
Message: 2
Date: Mon, 29 Aug 2011 13:24:00 -0400
From: Mary Helie <mary.helie <@t> yale.edu>
Subject: [Histonet] Thanks!
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4E5BCB30.5050400 <@t> yale.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Thank you all- this was very helpful. I appreciate it.
------------------------------
Message: 3
Date: Mon, 29 Aug 2011 14:04:00 -0400
From: "Carol Fields" <Carol.Fields <@t> Northside.com>
Subject: [Histonet] Cost of an H&E
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<731941C266951A47BEF11E5EFAAED9C90B666BFA <@t> nsmvexch01.northside.local>
Content-Type: text/plain;charset="US-ASCII"
Hi Netters,
If anyone has figured the cost of an H&E lately will you pleas share the
info with me? It has been awhile since I have done this. My email is
below if you do not mind sharing.
Thank you in advance.
Carole
Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
carol.fields <@t> northside.com
CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege.
------------------------------
Message: 4
Date: Mon, 29 Aug 2011 13:18:32 -0500
From: "Johnson, Teri" <TJJ <@t> stowers.org>
Subject: [Histonet] Re: iliac artery attachment to slides?
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2C40E43D1F7A56408C4463FD245DDDF97E107E28 <@t> EXCHMB-02.stowers-institute.org>
Content-Type: text/plain; charset="us-ascii"
Hi Jim, sounds like you are having a time of it.
I figure Jack Ratliff will chime in as soon as he sees this. In the meantime I will give you the same advice he gave to me.
If you are having troubles with tissues adhering, try Haupt's adhesive. You can find recipes on the internet to make it yourself, or you can buy it commercially ready to use (www.dornandhart.com). I have heard from several people who use this consistently with their MMA and they swear by it. Have you tried stretching the sections using a few drops of 50% alcohol and a couple of soft brushes prior to covering in plastic and clamping? It's going to be tough getting circular tissues wrinkle free. I hope others with more experience than me will chime in on this.
Best wishes,
Teri Johnson, HT(ASCP)QIHC
Head, Histology and Electron Microscopy
Stowers Institute for Medical Research
Kansas City, MO
------------------------------
Message: 5
Date: Mon, 29 Aug 2011 12:52:53 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Cost of an H&E
To: histonet <@t> lists.utsouthwestern.edu, Carol Fields
<Carol.Fields <@t> Northside.com>
Message-ID:
<1314647573.38427.YahooMailClassic <@t> web65701.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
The information on direct costs is in separate e-mail
René J.
--- On Mon, 8/29/11, Carol Fields <Carol.Fields <@t> Northside.com> wrote:
From: Carol Fields <Carol.Fields <@t> Northside.com>
Subject: [Histonet] Cost of an H&E
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, August 29, 2011, 2:04 PM
Hi Netters,
If anyone has figured the cost of an H&E lately will you pleas share the
info with me? It has been awhile since I have done this. My email is
below if you do not mind sharing.
Thank you in advance.
Carole
Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
carol.fields <@t> northside.com
CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege.
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Mon, 29 Aug 2011 15:36:01 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: [Histonet] training techs
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <885964FCAA9A431AB96C3DDC09E1D9EA <@t> Patsyoffice>
Content-Type: text/plain; charset="us-ascii"
Hey Everyone,
The third tech I trained in the last 3 years just passed her HTL exam last
Friday, that all makes me feel pretty good. I just signed an affiliate
agreement to be a clinical training site for the Histology program at UND
today, they are sending me another student who will be with me for 2
semesters.
Regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net
web site <http://www.ihctech.net> www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
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presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
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this e-mail as soon as possible.
------------------------------
Message: 7
Date: Mon, 29 Aug 2011 15:39:50 -0600
From: "gayle callis" <gayle.callis <@t> bresnan.net>
Subject: [Histonet] Charcoal filters for VIP1000 K series
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000f01cc6694$300ccd00$90266700$@bresnan.net>
Content-Type: text/plain; charset="us-ascii"
There are two charcoal filters (#6160) available for a Sakura Finetek VIP
1000, K series tissue processor. These are free for the lab who can use
them. Contact Maria Jerome [mjerome.mtedu <@t> gmail.com] for shipping
arrangements. She is not on Histonet.
------------------------------
Message: 8
Date: Mon, 29 Aug 2011 19:40:48 -0400
From: "histotech <@t> imagesbyhopper.com" <histotech <@t> imagesbyhopper.com>
Subject: Re: [Histonet] training techs
To: Patsy Ruegg <pruegg <@t> ihctech.net>
Cc: "<histonet <@t> lists.utsouthwestern.edu>"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <331D692F-049F-4BDD-B526-188536BC0F2F <@t> imagesbyhopper.com>
Content-Type: text/plain; charset=us-ascii
Way to go Patsy!! :o)
Sent from my iPhone
On Aug 29, 2011, at 5:36 PM, "Patsy Ruegg" <pruegg <@t> ihctech.net> wrote:
> Hey Everyone,
>
>
>
> The third tech I trained in the last 3 years just passed her HTL exam last
> Friday, that all makes me feel pretty good. I just signed an affiliate
> agreement to be a clinical training site for the Histology program at UND
> today, they are sending me another student who will be with me for 2
> semesters.
>
>
>
> Regards,
>
> Patsy
>
>
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> wk email <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net
> web site <http://www.ihctech.net> www.ihctech.net
>
>
>
>
> This email is confidential and intended solely for the use of the Person(s)
> ('the intended recipient') to whom it was addressed. Any views or opinions
> presented are solely those of the author. It may contain information that is
> privileged & confidential within the meaning of applicable law. Accordingly
> any dissemination, distribution, copying, or other use of this message, or
> any of its contents, by any person other than the intended recipient may
> constitute a breach of civil or criminal law and is strictly prohibited. If
> you are NOT the intended recipient please contact the sender and dispose of
> this e-mail as soon as possible.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 9
Date: Mon, 29 Aug 2011 19:47:50 -0400
From: "histotech <@t> imagesbyhopper.com" <histotech <@t> imagesbyhopper.com>
Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical
To: joelle weaver <joelleweaver <@t> hotmail.com>
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>,
"<madary <@t> verizon.net>" <madary <@t> verizon.net>
Message-ID: <98C4B261-D008-4D53-8DA0-887970D665BD <@t> imagesbyhopper.com>
Content-Type: text/plain; charset=us-ascii
Interestingly, I have never been required to section tissue in a job interview. I have worked at four hospitals, three private labs and one research facility (hubby moved us around a bit!)
I just recently hired two techs, directly out of school with no real world experience. I did ask them to cut some slides for me! ;o)
Michelle
Sent from my iPhone
On Aug 29, 2011, at 9:05 AM, joelle weaver <joelleweaver <@t> hotmail.com> wrote:
>
>
> I was required to know and understand all this as well. I often have had to perform various aspects on an interview, and had no problems with this.
> Joelle
>
> Joelle Weaver MAOM, BA, (HTL) ASCP
>
>
> From: b-frederick <@t> northwestern.edu
> To: lpwenk <@t> sbcglobal.net; madary <@t> verizon.net; histonet <@t> lists.utsouthwestern.edu
> Date: Mon, 29 Aug 2011 12:35:50 +0000
> Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical
> CC:
>
> I remember having to know, regarding Peggy's comment on why a reagent was on a piece of tissue, for my HTL what was going in every step of the retic (oxidation,reduction,toning etc) and believe me it was on the exam. We were taught to know the why from the techs that trained us and had taken the exam. If you chose to ignore what they said, it was on your head.
>
> As to the microtomy during an interview, I'm all for it as I have done it in the past (as an interviewer and interviewee) and most recently, as we had a tech come in from Romania and how were we to know what she knew? Their program is a CLS degree and she chose histo from that. Great tech by the way- histo is not much different the world over, from what I can see.
>
> Bernice
>
> Bernice Frederick HTL (ASCP)
> Senior Research Tech
> Pathology Core Facility
> ECOGPCO-RL
> Robert. H. Lurie Cancer Center
> Northwestern University
> 710 N Fairbanks Court
> Olson 8-421
> Chicago,IL 60611
> 312-503-3723
> b-frederick <@t> northwestern.edu
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk
> Sent: Sunday, August 28, 2011 6:41 PM
> To: madary <@t> verizon.net; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical
>
> Now add in a few other things, that I didn't directly mention in the original 2006 HistoNet reply.
>
> How many people are now using:
> - automated H&E stainers
> - automated special stainers, including IHC stainer
> - automated coverslippers
> - automated microtomes
> - disposable blades
>
> Can you see why nearly everyone passed the practical? If they could cut a half-way decent section (with better microtomes and blades, easier to do), just put the slide on a machine and let the machine stain it. To fail, they basically had to NOT follow a LOT of directions, such as submitting colon for small intestine, submitting autolyzed gall bladder, doing the wrong stain (like doing a Prussian blue for iron, instead of the requested colloidal iron), grossing the tissue too small, microtoming too thick, putting the institution's name on the label, etc. Automation makes it easier to produce better sections and better stains, particularly if someone is a mediocre tech to begin with.
>
> As to whether the person understands the theory when using automated stainers - well, the fact that many of the people submitting the practical could pass the practical but would fail the written - that has been going on since "ancient" times, when staining was done by hand. They could follow the directions, but didn't know the reasons. Pour on solution A for 5 minutes, pour it off, pour on solution B for 10 minutes, rinse it off, and it's done.
> No idea what is in solution A or B, or what chemicals are binding to what components in the tissue, etc. Whether someone is doing the stain by hand or by machine, it's up to the person to have the curiosity to find out what is going on. Some people don't have it, and don't feel the urge to learn.
>
> That's one of the reasons I like going to state and national meetings, and reading HistoNet. These people WANT to learn! Hurrah for them! And there are a lot of people in the histology community willing to help people who WANT to learn - answering HistoNet, giving talks at state and national meetings, being a mentor, etc. Hurrah for them too!
>
> Peggy A. Wenk, HTL(ASCP)SLS
> Beaumont Health Systems
> Royal Oak, MI 48073
>
> The above are my opinions and not those of my institution.
>
> -----Original Message-----
> From: madary <@t> verizon.net
> Sent: Sunday, August 28, 2011 2:23 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] peggy wenk comments on HT/HTL practical
>
>
> The dropping of the practical was explained so well by Peggy Wenk. I
> never got into the debate(glad I did not after reading Peggys
> comments), I would have argued to keep it. That said, indeed it is
> outdated. The fact that we were the last to do it I guess speaks
> volumes. I did struggle getting tissues for it for sure. Modern day,
> modern thinking. Thanks for the information Peggy and of course all
> you do fo this field.
>
> Nick(Rocky) Madary, HT/HTL(ASCP)QIHC
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________ Histonet mailing list Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 10
Date: Mon, 29 Aug 2011 20:15:11 -0400
From: "Jeff and Wanda Gray" <wgray19 <@t> sc.rr.com>
Subject: [Histonet] RE: Going for the HTL
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <005401cc66a9$e20e5cc0$a62b1640$@rr.com>
Content-Type: text/plain; charset="US-ASCII"
To Paula:
Who wrote, "thought it might be good to study and try for the HTL (already
have school for HT and am certified) but it looks like the time limits
prohibit me from trying this, since I did it so long ago. Anyone else have
any ideas? I'd gladly buy the books and study. Cannot get into a lab here in
NC without current experience. The closest school is in western NC, about 5
hours away. I didn't see anything near me in Raleigh. I was going to
purchase the textbooks but now I see this won't work either:"
There is an internet program just "down the road" in Charleston for HTLs:
www.musc.edu/histoprogram Our program is NACCLS approved, has start dates in
April and Sept. You can also call the director, Karen Brinker Geils @ (843)
792-4013. Take a look, I'll bet you can do it! It took me over 10 years, but
I got my BS and passed my HTL just this year! Life got in my way for a
while, but I did it, and I know you can too!
Wanda Shotsberger Gray
HT/HTL (ASCP), QIHC
------------------------------
Message: 11
Date: Mon, 29 Aug 2011 22:42:43 -0400
From: Bob Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: peggy wenk comments on HT/HTL practical
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAOKsRH4s8mv2xuRndsct3BQ98MW0LZhFV1vkbka1G3PN-COvag <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
I really appreciate Peggy Wenk's analysis of the practical examination
and why it had to be dropped. I never really understood the issue
before.
I must confess I always enjoyed helping the prospective examinee
obtain exactly the right tissue.
"No, this endometrium is poorly preserved. We'll arrange with surgery
for a completely fresh specimen - I'll block it initially for the
diagnosis, then we'll fix it overnight and then block it exactly to
specifications. - Ick - this one's been curetted - we'll get another
one".
"I'll block the margins of this colon resection specimen, then we'll
pin a portion of tissue onto paraffin and fix it flat overnight."
"Next time I do an autopsy we'll get a lumbar spinal cord in the
intact dura. I'll open the dura dorsally and ventrally with iridectomy
scissors, then we'll hang it in neutral buffered formalin for two
days. Then I'll tie the dura and dependent nerves with a cotton
string. When you embed you'll remove the string, taking care that dura
and nerves remain in position. After that it's all yours. If it
doesn't work the first time, we've got three more levels in the jar."
OK, I'm a geek, I'm 72 years old, I got a right.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 12
Date: Tue, 30 Aug 2011 11:13:45 +0200
From: Louise Renton <louise.renton <@t> gmail.com>
Subject: [Histonet] ACD RNAscope
To: Histonet <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CABL-0cA2V5bSdW0ocpM4Jw9dnsdb9T0D3-LoB_32O486u6H8xg <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi all - is anyone using the above system for RNA detection in FFPE tissues
- it sounds almost too good to be true?
--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
------------------------------
Message: 13
Date: Tue, 30 Aug 2011 03:12:40 -0700 (PDT)
From: Jean Brinker <j.brinker <@t> att.net>
Subject: [Histonet] supply inventory software
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1314699160.14821.YahooMailClassic <@t> web180314.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello,
Can anyone recommend a business accounting software to primarily track supply inventory/expenses? We are growing Urology practice (about to merge with another) and in need of a means of tracking for the lab internally. I appreciate your response.
Jean Brinker
Pathology Supervisor
St. Louis Urological Surgeons
St. Peters, MO 63376
------------------------------
Message: 14
Date: Tue, 30 Aug 2011 04:36:21 -0700 (PDT)
From: Sheila Haas <micropathlabs <@t> yahoo.com>
Subject: [Histonet] Slides
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1314704181.54150.YahooMailNeo <@t> web161704.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi all! We are having issues with the tissue falling off of our slides during routine
staining. The tissue primarily lifts from the bottom sections on the slides. We have
had our stainer checked and have checked ourselves, the oven appears to be
working properly and the water pressure is fine. I tried two different slide manufacturers, both silane coated, and are getting the same result. Not sure where to go at this point except to try a"non-coated" slide. Any suggestions on a manufacturer or vendor??
Thanks in advance. Your help is always appreciated!
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
------------------------------
Message: 15
Date: Tue, 30 Aug 2011 06:11:06 -0700 (PDT)
From: Pathology Lab <pathologylab <@t> ymail.com>
Subject: [Histonet] Looking for sponsors
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1314709866.51465.YahooMailNeo <@t> web121413.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi, im organizing a group of conference for the histotechs in Puerto Rico, and i would like to know if any company wants to come and share with us and sponsor the educational activity. Thanks and have a nice day !!!
Lcda. Mary V. Guerrero,BS, MBA,HtL
Administradora/Coordinadora General
Pathology Lab.
55 N. Dr. Basora Edificio Médico IV Oficina 206
Mayaguez, Puerto Rico 00680
Tel. 787-834-8202 Fax: 787-831-5255
Sra. Dimary Valentín Sra. Iris Franqui Sra. Myrna González
Facturación Reportes Transcripcion
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Message: 16
Date: Tue, 30 Aug 2011 15:15:08 +0200
From: Vanessa Orsini <vanessaorsini <@t> msn.com>
Subject: [Histonet] brazilin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <DUB108-W325096B6E40890AD0B1866B8170 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Hi everyone,
I'm Vanessa and i'm quite new in the histology field so i need some help...
i'm having problems with a LacZ staining on mouse fresh frozen tissue. I managed to get a nice staining also if I think it's a bit variable but I cannot find a good counterstain!!
I tried the nuclear fast red but it's not stable in an aqua-based mounting medium and if I do the acohol row before mounting with pertex the morphology of the sections is damaged and the lacz staining is a bit washed away (a problem if the Bgal expression is low)
I then tried with ematoxilin, the staining is stable but it's more difficult to discriminate between the blue of the lacZ and the blue of ematoxilin.
I find out that the brazilin is a red ematoxilin so i decided to try this one but once the powder arrived i didn't received any instruction for dilution with it I couldn't find anything on the net.
Does someone have experience with brazilin?
or do you know some other red counterstaining that I could use?
Thanks a lot
Vanessa
------------------------------
Message: 17
Date: Tue, 30 Aug 2011 06:33:07 -0700 (PDT)
From: Rob Geske <rgeske_2000 <@t> yahoo.com>
Subject: [Histonet] Capillary Gap Slides
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1314711187.18519.YahooMailClassic <@t> web39421.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
All,
can anyone suggest a source for all three sizes of capillary gap slides? Ventana, Fisher, and Cole-Palmer no longer stock them. thanks in advance.
rob
------------------------------
Message: 18
Date: Tue, 30 Aug 2011 09:36:23 -0400
From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
Subject: RE: [Histonet] Re: peggy wenk comments on HT/HTL practical
To: Bob Richmond <rsrichmond <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9BF995BC0E47744E9673A41486E24EE238DE5D0364 <@t> MERCERMAIL.MercerU.local>
Content-Type: text/plain; charset="us-ascii"
Not a Geek, just a good teacher.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Monday, August 29, 2011 10:43 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: peggy wenk comments on HT/HTL practical
I really appreciate Peggy Wenk's analysis of the practical examination
and why it had to be dropped. I never really understood the issue
before.
I must confess I always enjoyed helping the prospective examinee
obtain exactly the right tissue.
"No, this endometrium is poorly preserved. We'll arrange with surgery
for a completely fresh specimen - I'll block it initially for the
diagnosis, then we'll fix it overnight and then block it exactly to
specifications. - Ick - this one's been curetted - we'll get another
one".
"I'll block the margins of this colon resection specimen, then we'll
pin a portion of tissue onto paraffin and fix it flat overnight."
"Next time I do an autopsy we'll get a lumbar spinal cord in the
intact dura. I'll open the dura dorsally and ventrally with iridectomy
scissors, then we'll hang it in neutral buffered formalin for two
days. Then I'll tie the dura and dependent nerves with a cotton
string. When you embed you'll remove the string, taking care that dura
and nerves remain in position. After that it's all yours. If it
doesn't work the first time, we've got three more levels in the jar."
OK, I'm a geek, I'm 72 years old, I got a right.
Bob Richmond
Samurai Pathologist
Knoxville TN
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 19
Date: Tue, 30 Aug 2011 09:39:48 -0400
From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
Subject: RE: [Histonet] Slides
To: Sheila Haas <micropathlabs <@t> yahoo.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9BF995BC0E47744E9673A41486E24EE238DE5D0373 <@t> MERCERMAIL.MercerU.local>
Content-Type: text/plain; charset="iso-8859-1"
I use StayOn from Leica(Surgipath) which works really well on non-coated slides. I cut autopsy material, unusually large sections of bloody, dried, etc tissue and they stay on with StayOn. Gee I made a rhyme.
Shirley
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Tuesday, August 30, 2011 7:36 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Slides
Hi all! We are having issues with the tissue falling off of our slides during routine
staining. The tissue primarily lifts from the bottom sections on the slides. We have
had our stainer checked and have checked ourselves, the oven appears to be
working properly and the water pressure is fine. I tried two different slide manufacturers, both silane coated, and are getting the same result. Not sure where to go at this point except to try a"non-coated" slide. Any suggestions on a manufacturer or vendor??
Thanks in advance. Your help is always appreciated!
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 20
Date: Tue, 30 Aug 2011 10:19:17 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: [Histonet] Urgent - testing for cobalt in thyroid
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<92AD9B20A6C38C4587A9FEBE3A30E16408279EA9AD <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
Does anyone know what facility would do this? Please answer ASAP.. thanks!
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
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Message: 21
Date: Tue, 30 Aug 2011 09:29:42 -0500
From: <sdysart <@t> mirnarx.com>
Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical
To: <histotech <@t> imagesbyhopper.com>, <joelleweaver <@t> hotmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu, madary <@t> verizon.net
Message-ID:
<D957F2A7D21959488C492A2680F9920A2963CD <@t> SVREXCH.asuragen.us>
Content-Type: text/plain; charset="us-ascii"
I have been required to cut slides at every job interview I have ever
been to. I think this is good practice to follow because if you know
what you are doing you should be fine and have no worries. If you are a
book tech and have no practical experience then you should not be hired
over someone who does. I think the practical should come back as part
of the exam!! I had to do it...when you cut 900 slides to get 9 that
are perfect, you learn a thing or two on how to modify yourself to be
better!!
Just my two cents =)
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histotech <@t> imagesbyhopper.com
Sent: Monday, August 29, 2011 6:48 PM
To: joelle weaver
Cc: Histonet; <madary <@t> verizon.net>
Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical
Interestingly, I have never been required to section tissue in a job
interview. I have worked at four hospitals, three private labs and one
research facility (hubby moved us around a bit!)
I just recently hired two techs, directly out of school with no real
world experience. I did ask them to cut some slides for me! ;o)
Michelle
Sent from my iPhone
On Aug 29, 2011, at 9:05 AM, joelle weaver <joelleweaver <@t> hotmail.com>
wrote:
>
>
> I was required to know and understand all this as well. I often have
had to perform various aspects on an interview, and had no problems with
this.
> Joelle
>
> Joelle Weaver MAOM, BA, (HTL) ASCP
>
>
> From: b-frederick <@t> northwestern.edu
> To: lpwenk <@t> sbcglobal.net; madary <@t> verizon.net;
histonet <@t> lists.utsouthwestern.edu
> Date: Mon, 29 Aug 2011 12:35:50 +0000
> Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical
> CC:
>
> I remember having to know, regarding Peggy's comment on why a reagent
was on a piece of tissue, for my HTL what was going in every step of the
retic (oxidation,reduction,toning etc) and believe me it was on the
exam. We were taught to know the why from the techs that trained us and
had taken the exam. If you chose to ignore what they said, it was on
your head.
>
> As to the microtomy during an interview, I'm all for it as I have done
it in the past (as an interviewer and interviewee) and most recently,
as we had a tech come in from Romania and how were we to know what she
knew? Their program is a CLS degree and she chose histo from that. Great
tech by the way- histo is not much different the world over, from what I
can see.
>
> Bernice
>
> Bernice Frederick HTL (ASCP)
> Senior Research Tech
> Pathology Core Facility
> ECOGPCO-RL
> Robert. H. Lurie Cancer Center
> Northwestern University
> 710 N Fairbanks Court
> Olson 8-421
> Chicago,IL 60611
> 312-503-3723
> b-frederick <@t> northwestern.edu
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lee &
Peggy Wenk
> Sent: Sunday, August 28, 2011 6:41 PM
> To: madary <@t> verizon.net; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical
>
> Now add in a few other things, that I didn't directly mention in the
original 2006 HistoNet reply.
>
> How many people are now using:
> - automated H&E stainers
> - automated special stainers, including IHC stainer
> - automated coverslippers
> - automated microtomes
> - disposable blades
>
> Can you see why nearly everyone passed the practical? If they could
cut a half-way decent section (with better microtomes and blades, easier
to do), just put the slide on a machine and let the machine stain it. To
fail, they basically had to NOT follow a LOT of directions, such as
submitting colon for small intestine, submitting autolyzed gall bladder,
doing the wrong stain (like doing a Prussian blue for iron, instead of
the requested colloidal iron), grossing the tissue too small,
microtoming too thick, putting the institution's name on the label, etc.
Automation makes it easier to produce better sections and better stains,
particularly if someone is a mediocre tech to begin with.
>
> As to whether the person understands the theory when using automated
stainers - well, the fact that many of the people submitting the
practical could pass the practical but would fail the written - that has
been going on since "ancient" times, when staining was done by hand.
They could follow the directions, but didn't know the reasons. Pour on
solution A for 5 minutes, pour it off, pour on solution B for 10
minutes, rinse it off, and it's done.
> No idea what is in solution A or B, or what chemicals are binding to
what components in the tissue, etc. Whether someone is doing the stain
by hand or by machine, it's up to the person to have the curiosity to
find out what is going on. Some people don't have it, and don't feel the
urge to learn.
>
> That's one of the reasons I like going to state and national meetings,
and reading HistoNet. These people WANT to learn! Hurrah for them! And
there are a lot of people in the histology community willing to help
people who WANT to learn - answering HistoNet, giving talks at state and
national meetings, being a mentor, etc. Hurrah for them too!
>
> Peggy A. Wenk, HTL(ASCP)SLS
> Beaumont Health Systems
> Royal Oak, MI 48073
>
> The above are my opinions and not those of my institution.
>
> -----Original Message-----
> From: madary <@t> verizon.net
> Sent: Sunday, August 28, 2011 2:23 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] peggy wenk comments on HT/HTL practical
>
>
> The dropping of the practical was explained so well by Peggy Wenk.
I
> never got into the debate(glad I did not after reading
Peggys
> comments), I would have argued to keep it. That said, indeed it
is
> outdated. The fact that we were the last to do it I guess
speaks
> volumes. I did struggle getting tissues for it for sure. Modern
day,
> modern thinking. Thanks for the information Peggy and of course
all
> you do fo this field.
>
> Nick(Rocky) Madary, HT/HTL(ASCP)QIHC
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________ Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 22
Date: Tue, 30 Aug 2011 14:38:26 +0000
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
Subject: RE: [Histonet] Slides
To: Sheila Haas <micropathlabs <@t> yahoo.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AEE9D3B1BA593A49B23DA5A6A375654C3ED0ADE4 <@t> EX2010-01.barrynet.barry.edu>
Content-Type: text/plain; charset="us-ascii"
Try coating the slides yourself with Vector Labs "Vectabond." Neither I nor any of my graduate students have ever lost a section from a slide coated with "Vectabond."
-Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Podiatric Medicine
Miami Shores, Florida
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Sheila Haas [micropathlabs <@t> yahoo.com]
Sent: Tuesday, August 30, 2011 7:36 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Slides
Hi all! We are having issues with the tissue falling off of our slides during routine
staining. The tissue primarily lifts from the bottom sections on the slides. We have
had our stainer checked and have checked ourselves, the oven appears to be
working properly and the water pressure is fine. I tried two different slide manufacturers, both silane coated, and are getting the same result. Not sure where to go at this point except to try a"non-coated" slide. Any suggestions on a manufacturer or vendor??
Thanks in advance. Your help is always appreciated!
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 23
Date: Tue, 30 Aug 2011 11:36:36 -0400
From: "Heath, Nancy L." <NHeath <@t> Lifespan.org>
Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical
To: <sdysart <@t> mirnarx.com>, <histotech <@t> imagesbyhopper.com>,
<joelleweaver <@t> hotmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu, madary <@t> verizon.net
Message-ID:
<130E8991F210424096EFC6F42EA33B2408043949 <@t> LSCOEXCH1.lsmaster.lifespan.org>
Content-Type: text/plain; charset="us-ascii"
I totally agree Sarah :)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
sdysart <@t> mirnarx.com
Sent: Tuesday, August 30, 2011 10:30 AM
To: histotech <@t> imagesbyhopper.com; joelleweaver <@t> hotmail.com
Cc: histonet <@t> lists.utsouthwestern.edu; madary <@t> verizon.net
Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical
I have been required to cut slides at every job interview I have ever
been to. I think this is good practice to follow because if you know
what you are doing you should be fine and have no worries. If you are a
book tech and have no practical experience then you should not be hired
over someone who does. I think the practical should come back as part
of the exam!! I had to do it...when you cut 900 slides to get 9 that
are perfect, you learn a thing or two on how to modify yourself to be
better!!
Just my two cents =)
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histotech <@t> imagesbyhopper.com
Sent: Monday, August 29, 2011 6:48 PM
To: joelle weaver
Cc: Histonet; <madary <@t> verizon.net>
Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical
Interestingly, I have never been required to section tissue in a job
interview. I have worked at four hospitals, three private labs and one
research facility (hubby moved us around a bit!)
I just recently hired two techs, directly out of school with no real
world experience. I did ask them to cut some slides for me! ;o)
Michelle
Sent from my iPhone
On Aug 29, 2011, at 9:05 AM, joelle weaver <joelleweaver <@t> hotmail.com>
wrote:
>
>
> I was required to know and understand all this as well. I often have
had to perform various aspects on an interview, and had no problems with
this.
> Joelle
>
> Joelle Weaver MAOM, BA, (HTL) ASCP
>
>
> From: b-frederick <@t> northwestern.edu
> To: lpwenk <@t> sbcglobal.net; madary <@t> verizon.net;
histonet <@t> lists.utsouthwestern.edu
> Date: Mon, 29 Aug 2011 12:35:50 +0000
> Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical
> CC:
>
> I remember having to know, regarding Peggy's comment on why a reagent
was on a piece of tissue, for my HTL what was going in every step of the
retic (oxidation,reduction,toning etc) and believe me it was on the
exam. We were taught to know the why from the techs that trained us and
had taken the exam. If you chose to ignore what they said, it was on
your head.
>
> As to the microtomy during an interview, I'm all for it as I have done
it in the past (as an interviewer and interviewee) and most recently,
as we had a tech come in from Romania and how were we to know what she
knew? Their program is a CLS degree and she chose histo from that. Great
tech by the way- histo is not much different the world over, from what I
can see.
>
> Bernice
>
> Bernice Frederick HTL (ASCP)
> Senior Research Tech
> Pathology Core Facility
> ECOGPCO-RL
> Robert. H. Lurie Cancer Center
> Northwestern University
> 710 N Fairbanks Court
> Olson 8-421
> Chicago,IL 60611
> 312-503-3723
> b-frederick <@t> northwestern.edu
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lee &
Peggy Wenk
> Sent: Sunday, August 28, 2011 6:41 PM
> To: madary <@t> verizon.net; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical
>
> Now add in a few other things, that I didn't directly mention in the
original 2006 HistoNet reply.
>
> How many people are now using:
> - automated H&E stainers
> - automated special stainers, including IHC stainer
> - automated coverslippers
> - automated microtomes
> - disposable blades
>
> Can you see why nearly everyone passed the practical? If they could
cut a half-way decent section (with better microtomes and blades, easier
to do), just put the slide on a machine and let the machine stain it. To
fail, they basically had to NOT follow a LOT of directions, such as
submitting colon for small intestine, submitting autolyzed gall bladder,
doing the wrong stain (like doing a Prussian blue for iron, instead of
the requested colloidal iron), grossing the tissue too small,
microtoming too thick, putting the institution's name on the label, etc.
Automation makes it easier to produce better sections and better stains,
particularly if someone is a mediocre tech to begin with.
>
> As to whether the person understands the theory when using automated
stainers - well, the fact that many of the people submitting the
practical could pass the practical but would fail the written - that has
been going on since "ancient" times, when staining was done by hand.
They could follow the directions, but didn't know the reasons. Pour on
solution A for 5 minutes, pour it off, pour on solution B for 10
minutes, rinse it off, and it's done.
> No idea what is in solution A or B, or what chemicals are binding to
what components in the tissue, etc. Whether someone is doing the stain
by hand or by machine, it's up to the person to have the curiosity to
find out what is going on. Some people don't have it, and don't feel the
urge to learn.
>
> That's one of the reasons I like going to state and national meetings,
and reading HistoNet. These people WANT to learn! Hurrah for them! And
there are a lot of people in the histology community willing to help
people who WANT to learn - answering HistoNet, giving talks at state and
national meetings, being a mentor, etc. Hurrah for them too!
>
> Peggy A. Wenk, HTL(ASCP)SLS
> Beaumont Health Systems
> Royal Oak, MI 48073
>
> The above are my opinions and not those of my institution.
>
> -----Original Message-----
> From: madary <@t> verizon.net
> Sent: Sunday, August 28, 2011 2:23 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] peggy wenk comments on HT/HTL practical
>
>
> The dropping of the practical was explained so well by Peggy Wenk.
I
> never got into the debate(glad I did not after reading
Peggys
> comments), I would have argued to keep it. That said, indeed it
is
> outdated. The fact that we were the last to do it I guess
speaks
> volumes. I did struggle getting tissues for it for sure. Modern
day,
> modern thinking. Thanks for the information Peggy and of course
all
> you do fo this field.
>
> Nick(Rocky) Madary, HT/HTL(ASCP)QIHC
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________ Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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End of Histonet Digest, Vol 93, Issue 41
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