[Histonet] ACD RNAscope

Kim Merriam kmerriam2003 <@t> yahoo.com
Tue Aug 30 12:29:04 CDT 2011

I have been trying to get it to work using their actin (and control probes) probe on some routine FFPE cell pellets that I had, and I have not had any luck at all with it.  Truly, the kit is a breeze to use, but I can't get the darn thing to work.  I have had some very modest success with their kit on frozen sections.
I have shipped them some of my slides and they have agreed to test them in their lab. 
Let me know if you have any success!


Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA

From: Louise Renton <louise.renton <@t> gmail.com>
To: Histonet <Histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, August 30, 2011 5:13 AM
Subject: [Histonet] ACD RNAscope

Hi all - is anyone using the above system for RNA detection in FFPE tissues
- it sounds almost too good to be true?

Louise Renton
Bone Research Unit
University of the Witwatersrand
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
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