[Histonet] Re: iliac artery attachment to slides?

[Johnson, Teri]

Hi Jim, sounds like you are having a time of it.

I figure Jack Ratliff will chime in as soon as he sees this. In the meantime I will give you the same advice he gave to me.

If you are having troubles with tissues adhering, try Haupt's adhesive. You can find recipes on the internet to make it yourself, or you can buy it commercially ready to use (www.dornandhart.com). I have heard from several people who use this consistently with their MMA and they swear by it. Have you tried stretching the sections using a few drops of 50% alcohol and a couple of soft brushes prior to covering in plastic and clamping? It's going to be tough getting circular tissues wrinkle free. I hope others with more experience than me will chime in on this.

Best wishes,

Teri Johnson, HT(ASCP)QIHC
Head, Histology and Electron Microscopy
Stowers Institute for Medical Research
Kansas City, MO