[Histonet] instructions for steaming slides

[Kim Merriam]

We use a black and decker rice steamer fairly routinely.  It has the ability to steam for a total of 75 minutes, although it takes a good 15 of them to get up to temperature.  I like it becasue the temperature is nice and even and it is very easy to do.
	1. add water to the bottom of the steamer and turn it on (I turn the dial all the way to 75 minutes)
	2. preheate the HIER solution in the microwave (90-120 seconds for a tissue-tek staining bucket is fine)
	3. add slides to the solution and place into the top compartment of the steamer
	4. steam for desired time (in tests that I did many years ago, most antibodies required a minimum of 45 minutes in the steamer)
	5. you can check the temperature of your retrieval solution, it should be about 100C
	6. once done, remove, cool, rinse in water and proceed with your staining
Good luck!
Kim


Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA

From: "Kant, H.J.G. van de (Henk)" <H.J.G.vandeKant <@t> uu.nl>
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, August 25, 2011 1:49 AM
Subject: [Histonet] instructions for steaming slides

Dear All,

Can somebody help me with instructions (protocol) for steaming slides in a domestic ricecooker or vegetable steamer.
Head induced antigen retrieval method is with sodium citrate 0.01 M pH 6.0.



Kind regards,



Henk van de Kant

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