[Histonet] RE: In situ on paraffin sections - specifically bone
Timothy.Morken <@t> ucsfmedctr.org
Wed Aug 24 10:24:11 CDT 2011
Anna, one issue is the decal method. Rapid acid decal can lead to non-specific staining due to extraction of nuclear proteins. Most ISH decal methods recommend EDTA. It is much slower but does not cause the problem.
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hughes, Anna
Sent: Wednesday, August 24, 2011 7:55 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] In situ on paraffin sections - specifically bone
I am wondering if anyone out there has any experience doing FISH or ISH on paraffin sections - either automated or not (We have a Ventana and are acquiring a BioGenex Xmatrx)- but I am specifically interested in doing it in paraffin embedded bone tissue. I have pulled a few publications from labs that are doing IHC and FISH on the same paraffin section, but have mostly seen this done in tumors, not in bone.
Any information or direction would be appreciated! Thanks!
Anna C. Hughes
Merck & Co., Inc.
anna_hughes <@t> merck.com
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