[Histonet] RE: In situ on paraffin sections - specifically bone

[Morken, Timothy]

Anna, one issue is the decal method. Rapid acid decal can lead to non-specific staining due to extraction of nuclear proteins. Most ISH decal methods recommend EDTA. It is much slower but does not cause the problem. 

Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hughes, Anna
Sent: Wednesday, August 24, 2011 7:55 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] In situ on paraffin sections - specifically bone

Hi Everyone!

I am wondering if anyone out there has any experience doing FISH or ISH on paraffin sections - either automated or not (We have a Ventana and are acquiring a BioGenex Xmatrx)- but I am specifically interested in doing it in paraffin embedded bone tissue.  I have pulled a few publications from labs that are doing IHC and FISH on the same paraffin section, but have mostly seen this done in tumors, not in bone.

Any information or direction would be appreciated!  Thanks!

Anna Hughes
Anna C. Hughes
Merck & Co., Inc.
anna_hughes <@t> merck.com



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