[Histonet] RE: melanin counterstaining
solsen <@t> MPLNet.com
Wed Aug 17 13:56:51 CDT 2011
You can try a simple Schmorl's stain - which stains melanin. You can find it in a Sheehan/Shrapchek
Steven K. Olsen
Chief Operating Officer, Director of Anatomic Pathology
Molecular Pathology Laboratory Network, Inc.
250 E. Broadway, Maryville, TN 37804
P 865.380.9746 ext.187
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From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira
Sent: Wednesday, August 17, 2011 2:51 PM
To: histonet <@t> lists.utsouthwestern.edu; histonet-request <@t> lists.utsouthwestern.edu
Subject: [Histonet] melanin counterstaining
I have heavily pigmented melanocytic cells and trying to differentiate between melanin pigment and chromogen (DAB) by using Azure B.
After IHC with DAB I incubate slides for 30-45 min in the working solution of:
* 4ml of a 25mg/ml aqueous solution of Azure B
* 3.4ml of 0.1M acetic acid
* 600µl of 0.1M sodium acetate
* 27ml of DI water
* 5ml acetone
Then water wash and hematoxylin counter-stain. By the end all melanin supposed to turn blue-green, but I still see some remained brown melanin.
My question is: I would like to increase green color, so the concentration of what reagent I have to increase?
Thanks in advance,
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