[Histonet] RE: melanin counterstaining

[Steve Olsen]

You can try a simple Schmorl's stain - which stains melanin.  You can find it in a Sheehan/Shrapchek


Steven K. Olsen
Chief Operating Officer, Director of Anatomic Pathology
Molecular Pathology Laboratory Network, Inc.
250 E. Broadway, Maryville, TN 37804

P  865.380.9746 ext.187
C  865.978.0321
F  865.380.9191

http://www.MPLNET.com/
http://www.Geneuity.com/

Confidentiality Notice The information in this communication is confidential and intended for the exclusive use of the designated addressee. If you are not the addressee, any disclosure, reproduction or distribution of this communication and/or any attachments is strictly prohibited. If you are a covered entity and entitled to the information please notify us if there are exposures to the information contained in this communication as required by HIPAA and HITEC. If you received this communication in error, please notify the sender immediately.


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira
Sent: Wednesday, August 17, 2011 2:51 PM
To: histonet <@t> lists.utsouthwestern.edu; histonet-request <@t> lists.utsouthwestern.edu
Subject: [Histonet] melanin counterstaining

Good morning,

I have heavily pigmented melanocytic cells and trying to differentiate between melanin pigment and chromogen (DAB) by using Azure B.

After IHC with DAB I incubate slides for 30-45 min in the working solution of:

 *   4ml of a 25mg/ml aqueous solution of Azure B
 *   3.4ml of 0.1M acetic acid
 *   600µl of 0.1M sodium acetate
 *   27ml of DI water
 *   5ml acetone

Then water wash and hematoxylin counter-stain. By the end all melanin supposed to turn blue-green, but I still see some remained brown melanin.

My question is: I would like to increase green color, so the concentration of what reagent I have to increase?

Thanks in advance,
Naira

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet