[Histonet] Frozen Tissue Protocol

Sheila Fonner sfonner <@t> labpath.com
Wed Aug 17 12:12:24 CDT 2011



Where would I insert this step in my protocol if I am staining on the
Ventana Ultra?  Can I do it by hand after the slides are finished?  Thanks
for your help.





From: Paula Pierce [mailto:contact <@t> excaliburpathology.com] 
Sent: Wednesday, August 17, 2011 11:14 AM
To: Sheila Fonner
Subject: Re: [Histonet] Frozen Tissue Protocol


You are seeing endogenous phosphatase, not endogenous peroxidase. Use 10%
acetic acid for 1-2 minutes.

Paula K. Pierce, HTL(ASCP)HT


Excalibur Pathology, Inc.

631 N Broadway

Moore, OK 73160

405-759-3953 Lab

405-759-7513 Fax

www.excaliburpathology.com <http://www.excaliburpathology.com/> 




From: Sheila Fonner <sfonner <@t> labpath.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wed, August 17, 2011 9:45:18 AM
Subject: [Histonet] Frozen Tissue Protocol

Hi everyone,

Yesterday I asked for help with modifying my protocols to stain HSVI, HSVII,
and Zoster.  I appreciate everyone's help.  Now I am back for more info.  I
deleted the depar. and cell conditioning steps and I got specific staining
with little background.  There was just one problem.the pathologist says
that the leukocytes are reacting with the alk. phos on all of the slides.
Does anyone know if a blocking step would help in this case?  Just a
reminder - I am using the Ventana Ultra platform with Ultraview Red
Detection.  Thanks again Histoland.  You never let me down! J


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