[Histonet] H&E on Frozen

Reynolds,Donna M dreynold <@t> mdanderson.org
Tue Aug 16 09:13:06 CDT 2011

We do this frequently. 
Fix slides in Methanol (45ml) + formaldehyde (5ml) for 10 minutes. 
Wash several changes of tap water
Stain with Harris Hematoxylin for 3 minutes
Proceed as you would for paraffin  sections
Get great staining. 
I am sure if you are using a different Hematoxylin you could use it the same way you use it for paraffin

Donna Reynolds HT(ASCP)
Chief Histology Technician, Core Immuno Lab
U. T. M.D. Anderson Cancer Center, Houston, TX
e-mail dreynold <@t> mdanderson.org

Message: 2
Date: Fri, 12 Aug 2011 08:52:11 -0700 (PDT)
From: Jennifer Sipes <jengirl1014 <@t> yahoo.com>
Subject: [Histonet] H&E for Frozen Tissue
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
	<1313164331.88113.YahooMailNeo <@t> web125406.mail.ne1.yahoo.com>
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Does anyone have a good protocol for staining frozen sections with H&E?? Also, any mounting medium suggestions?? This is the first time I've been asked to do this for frozen. 
Thanks a bunch everyone!
Jennifer K. Sipes, ALAT 
Sr. Laboratory Technician 
Johns Hopkins University 
Ross 933 
720 Rutland Avenue 
Baltimore, MD? 21205 
phone:???? 410-614-0131 
fax:???????? 410-955-9677 
cell:???????? 443-631-6361 
e-mail:? jsipes1 <@t> jhmi.edu 



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