[Histonet] H&E on Frozen
dreynold <@t> mdanderson.org
Tue Aug 16 09:13:06 CDT 2011
We do this frequently.
Fix slides in Methanol (45ml) + formaldehyde (5ml) for 10 minutes.
Wash several changes of tap water
Stain with Harris Hematoxylin for 3 minutes
Proceed as you would for paraffin sections
Get great staining.
I am sure if you are using a different Hematoxylin you could use it the same way you use it for paraffin
Donna Reynolds HT(ASCP)
Chief Histology Technician, Core Immuno Lab
U. T. M.D. Anderson Cancer Center, Houston, TX
e-mail dreynold <@t> mdanderson.org
Date: Fri, 12 Aug 2011 08:52:11 -0700 (PDT)
From: Jennifer Sipes <jengirl1014 <@t> yahoo.com>
Subject: [Histonet] H&E for Frozen Tissue
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
<1313164331.88113.YahooMailNeo <@t> web125406.mail.ne1.yahoo.com>
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Does anyone have a good protocol for staining frozen sections with H&E?? Also, any mounting medium suggestions?? This is the first time I've been asked to do this for frozen.
Thanks a bunch everyone!
Jennifer K. Sipes, ALAT
Sr. Laboratory Technician
Johns Hopkins University
720 Rutland Avenue
Baltimore, MD? 21205
e-mail:? jsipes1 <@t> jhmi.edu
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