[Histonet] Re: IHC on Pig help

[shive003 <@t> umn.edu]

Carl is correct... treat the tissue as if it is human... i.e., use 
anti-mouse IgG as your link secondary Ab for CD68 mouse primary antibody, 
etc. Not all CD68s cross-react with non-primates; I can let you know which 
clones I've tried when I get back into work on Monday.

Jan Shivers
IHC Section Head
Veterinary Diagnostic Lab
Univ. of Minnesota College of Veterinary Medicine
St. Paul, MN 

On Aug 13 2011, Hobbs, Carl wrote:

>
>Treat the tissue exactly as if it was human.
>
>Sure, your anti CD68 may not work on pig but, it's worth a try.
>LBH? Probably LDH. If so, better to measure serum levels?
>Trypen blue? Prob. Trypan blue. It's a dye used for cell viability tests.
>
>So, it seems that 2 and 3 are not IHC at all.
>
>More details?
>Also ask for IHC help here, if you wish : http://www.immunoportal.com/
>
>
>Carl Hobbs
>Histology Manager
>Wolfson CARD
>School of Biomedical Sciences
>Kings College London
>Guys Campus
>SE1 1UL
>Tel: 020 78486813
>Fax: 020 78486816
>020 78486813
>
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