[Histonet] sterilized cassette
Lyn Stadler
LStadler <@t> cbiolabs.com
Fri Aug 12 08:49:57 CDT 2011
Have you tried the "cell safe" cassette insert from Ted Pella?
http://www.tedpella.com/embed_html/27141.htm#anchor27165
Cellsafe(tm) Biopsy Cassettes (white) shown open and closed & inserted into a regular tissue cassette.
Cellsafe(tm) Biopsy Cassettes
The Cellsafe(tm) is designed to contain small tissue fragments and biopsies during tissue processing. The Cellsafe(tm) Biopsy Insert with a hinged cover fits into a regular cassette. Top and bottom consist of extra fine nylon mesh preventing small tissues from getting lost during specimen preparation, keeping them safe. The plastic frame and nylon mesh are suitable for use in common tissue processing reagents including Formaldehyde solutions, Bouin's Fluid, Alcohol solutions, Xylene, Chloroform, Citrus based clearing agents and Paraffin wax (melting point up to 65°C). The plastic frame is made from the same material widely used in the manufacture of plastic processing cassettes. If you are using any unusual reagents it is suggested that you carry out preliminary chemical compatibility tests before using the Cellsafe(tm) to contain tissue samples. The Cellsafe(tm) is designed for single use only.
Interior dimesions of a regular cassette are: 30 x 26 x 4.92mm H.
Exterior dimensions of the Cellsafe Biopsy Cassettes are: 27.5 x 25 x 4.57mm H.
Item # 21765, bag of 100 $40.85
Lyn Stadler
Histology Technician
Department of Histopathology
Cleveland Biolabs, Inc.
73 High Street
Buffalo, NY 14203
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, August 10, 2011 1:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 93, Issue 13
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Today's Topics:
1. RE: TFE-3 (Bernice Frederick)
2. What's your favorite phospho-histone H3 antibody for IHC?
(Connolly, Brett M)
3. Re: Manual embedding (Collette, Nicole M.)
4. Fern tissue loss during in situ hybridization (Cordle, Angela R)
5. Re: Fern tissue loss during in situ hybridization (Rene J Buesa)
6. Sterilized cassette (Brandi Farris)
7. Re:2 (lynn13361 <@t> aol.com)
8. RE: Sterilized cassette (Mike Pence)
9. Cell block H&E staining (Dessoye, Michael J)
10. Re: Cell block H&E staining (Rene J Buesa)
11. Histotech needed in San Diego. Can you help? (Pam Barker)
12. CYTOLOGIST JOB OPENING PHILLY (rmweber113 <@t> comcast.net)
13. GROSSING SPECIMENS (Sara Baldwin/mhhcc.org)
14. Cyrostat Oppinions (McMahon, Loralee A)
----------------------------------------------------------------------
Message: 1
Date: Tue, 9 Aug 2011 18:05:51 +0000
From: Bernice Frederick <b-frederick <@t> northwestern.edu>
Subject: [Histonet] RE: TFE-3
To: "Settembre, Dana" <settembr <@t> umdnj.edu>, "'Houston, Ronald'"
<Ronald.Houston <@t> nationwidechildrens.org>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<62C639732D3F274DACED033EBDF6ADAF1E12D90D <@t> evcspmbx3.ads.northwestern.edu>
Content-Type: text/plain; charset="us-ascii"
Same here.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Settembre, Dana
Sent: Tuesday, August 09, 2011 11:05 AM
To: 'Houston, Ronald'; 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: TFE-3
We use TFE-3 (p16) from Santa Cruz, made in Goat. Cat.# SC-5958
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Houston, Ronald
Sent: Tuesday, August 09, 2011 12:02 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] TFE-3
Is anyone using TFE-3? If so I would appreciate which clone is being used most.
Thanks
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital www.childlab.com
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston <@t> nationwidechildrens.org<mailto:ronald.houston <@t> nationwidechildrens.org>
www.NationwideChildrens.org<http://www.NationwideChildrens.org>
"One person with passion is better than forty people merely interested."
~ E.M. Forster
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------------------------------
Message: 2
Date: Tue, 9 Aug 2011 15:09:47 -0400
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Subject: [Histonet] What's your favorite phospho-histone H3 antibody
for IHC?
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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<C01C35B84DCDCE49BC60867E87F1C8FE3370A9ACE4 <@t> USCTMXP51015.merck.com>
Content-Type: text/plain; charset="us-ascii"
....p(Ser10) or p(Ser28) ?
And have you found pHH3 (Ser28) to be more M-phase specific?
Thanks,
Brett
Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803
Notice: This e-mail message, together with any attachments, contains
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Message: 3
Date: Tue, 9 Aug 2011 12:10:24 -0700
From: "Collette, Nicole M." <collette2 <@t> llnl.gov>
Subject: Re: [Histonet] Manual embedding
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <CA66D430.70A2%collette2 <@t> llnl.gov>
Content-Type: text/plain; charset="us-ascii"
Hi, All,
I just saw this question and the responses, thought I would add my own
solution to the mix. I do manual embedding, I use a hyb oven for my
infiltrating/embedding station (like for hybridizing Southern or Northern
blots- who does that anymore?). I have taken out the rotating wheel for
spinning bottles, and line the tray at the bottom with foil. Temp control
works very well. I have several Wheaton staining boxes (the kind that come
with glass inserts for staining 20 slides) that I use for my wax changes for
infiltrating, and a couple of metal beakers I use for pouring into molds. I
have a little real estate left inside the oven (it's probably around 18"x
18" square area) to heat my molds for pouring, and I have a metal heat block
in there that you would use for Eppendorf tubes as my forceps warmer. It
works pretty well, but does take a long time to heat up those boxes of wax,
so I need to plan ahead by about 3 hours. When I pour the molds, I have the
oven in front of a drawer under the bench, I line an extra cabinet shelf
with foil and lay it over the open drawer to give me some bench space, pour
my molds inside the oven and transfer it to the foil-lined shelf to cool so
I can move it without the specimen shifting. Then I transfer to a photo tray
and cool in the fridge for a couple of hours before releasing the molds.
With the door of the hyb oven open I have to embed in shifts and let the
molds heat up again, but it works OK. At least it's all contained in one
unit. It's hard to do histology in a molecular lab ;)
Hope this helps to give a do-it-your-selfer some ideas.
Sincerely,
Nicole Collette
LLNL
On 8/2/11 7:53 PM, "Scott Parker" <sparker <@t> vt.edu> wrote:
> Dear Histonetters:
>
> I am interested in acquiring a pitcher and heating jacket for melting and
> pouring paraffin during manual embedding. My work is relatively low volume
> and in a university research lab setting so I am trying to avoid purchasing
> an expensive embedding station. Can anyone recommend an honest supplier of
> used histology equipment that might be able to provide me with this item?
>
> Thank you for your expertise!
>
> Scott L. Parker
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Tue, 9 Aug 2011 20:46:03 +0000
From: "Cordle, Angela R" <angela-cordle <@t> uiowa.edu>
Subject: [Histonet] Fern tissue loss during in situ hybridization
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<32F6F3C172009D44A3B5E6702B54DBF602A772D2 <@t> itsnt443.iowa.uiowa.edu>
Content-Type: text/plain; charset="Windows-1252"
Dear Histonet members,
I?m having problems with tissue loss during in situ hybridization (ISH) with fern tissue. The fern in question is Ceratopteris richardii, and the tissue (tissue does not contain spores) has been fixed in FAA, dehydrated and embedded in Paraplast plus. Sections are 7 um, and mounted on Probe on Plus slides. I?m not an expert at this process, but I?ve done a fair amount of embedding, sectioning, and staining of various tissues (on Haupt?s coated slies), and I?ve had success with Arabidpsis ISH. I?ll outline the relevant and troubleshooting procedures that I?ve gone through here:
1. Varied the fixation times and methods. Nothing seems to make a difference in the retention of the tissue on the slides.
2. Tried a brand new box of Superfrost Plus slides (the ProbeOn Plus slides are kind-of old), but the superfrost slides actually seemed to provide worse tissue retention than the ProbeOn Plus slides. Arabidopsis tissue prepared in parallel was fine on both slides.
3. For mounting, I float tissue on 37?C DEPC H2O in a waterbath that is free from lotions, or any other substance that I am aware of that can cause problems with tissue adherence. I remove the slides with the newly adhered sections completely vertically. Again, Arabidopsis sections mounted in parallel do not fall off slides in subsequent procedures.
4. I?ve paid a (perhaps) compulsive amount of time making sure that there are no blebs or bubbles of water underneath sections after mounting. Additionally, slides are dried for 1-2 hours (vertically) before baking them at 48?C. I have found that baking the slides for 2 days, rather than 1, slightly increases the tissue retention for the fern, but not enough for quality in situ results.
5. Varied the time and temperature of the proteinase K treatment, but the tissue seems to be falling off the slides before this step anyway. It is gone after this step for sure, regardless of the time or temperature.
5. Taken a great amount of care to ensure that there is no residual tert butyl alcohol (we dehydrate through an ethanol series them into 100% TBA before transitioning to Paraplast) in the paraplast before embedding and sectioning. Arabidopsis tissue embedded in the same blocks has performed perfectly fine in ISH.
Okay. So, here are my specific questions:
Could it be that this fern tissue is not sufficiently negatively charged that the tissue will not adhere to any polyL lysine coated slide?
What the beep should I try next?
In advance, thank you all so much for taking the time to help me out with this frustrating problem! --Angie
------------------------------
Message: 5
Date: Tue, 9 Aug 2011 13:59:18 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Fern tissue loss during in situ hybridization
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, Angela RCordle
<angela-cordle <@t> uiowa.edu>
Message-ID:
<1312923558.59874.YahooMailClassic <@t> web65711.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=utf-8
I think you should try to find out about the cellulose contents of the fern you are working with, and compare it to that of Arabidopsis. If the fern (Ceratopteris) contains more cellulose, I think this is the cause of your problem.
If that is the case I think you should try to use a paraffin wax of higher melting point (63-65??C) to assure an infiltration more compatible with the cellulose. Also make sure that the infiltration is optimal.
Finally if you can use thinner sections (<7 ??m) that could help also.
Ren?? J.
--- On Tue, 8/9/11, Cordle, Angela R <angela-cordle <@t> uiowa.edu> wrote:
From: Cordle, Angela R <angela-cordle <@t> uiowa.edu>
Subject: [Histonet] Fern tissue loss during in situ hybridization
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Tuesday, August 9, 2011, 4:46 PM
Dear Histonet members,
I???m having problems with tissue loss during in situ hybridization (ISH) with fern tissue. The fern in question is Ceratopteris richardii, and the tissue (tissue does not contain spores) has been fixed in FAA, dehydrated and embedded in Paraplast plus. Sections are 7 um, and mounted on Probe on Plus slides. I???m not an expert at this process, but I???ve done a fair amount of embedding, sectioning, and staining of various tissues (on Haupt???s coated slies), and I???ve had success with Arabidpsis ISH. I???ll outline the relevant and troubleshooting procedures that I???ve gone through here:
1. Varied the fixation times and methods. Nothing seems to make a difference in the retention of the tissue on the slides.
2. Tried a brand new box of Superfrost Plus slides (the ProbeOn Plus slides are kind-of old), but the superfrost slides actually seemed to provide worse tissue retention than the ProbeOn Plus slides. Arabidopsis tissue prepared in parallel was fine on both slides.
3. For mounting, I float tissue on 37??C DEPC H2O in a waterbath that is free from lotions, or any other substance that I am aware of that can cause problems with tissue adherence. I remove the slides with the newly adhered sections completely vertically. Again, Arabidopsis sections mounted in parallel do not fall off slides in subsequent procedures.
4. I???ve paid a (perhaps) compulsive amount of time making sure that there are no blebs or bubbles of water underneath sections after mounting. Additionally, slides are dried for 1-2 hours (vertically) before baking them at 48??C. I have found that baking the slides for 2 days, rather than 1, slightly increases the tissue retention for the fern, but not enough for quality in situ results.
5. Varied the time and temperature of the proteinase K treatment, but the tissue seems to be falling off the slides before this step anyway. It is gone after this step for sure, regardless of the time or temperature.
5. Taken a great amount of care to ensure that there is no residual tert butyl alcohol (we dehydrate through an ethanol series them into 100% TBA before transitioning to Paraplast) in the paraplast before embedding and sectioning. Arabidopsis tissue embedded in the same blocks has performed perfectly fine in ISH.
Okay. So, here are my specific questions:
Could it be that this fern tissue is not sufficiently negatively charged that the tissue will not adhere to any polyL lysine coated slide?
What the beep should I try next?
In advance, thank you all so much for taking the time to help me out with this frustrating problem!?? ?? --Angie
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Tue, 9 Aug 2011 19:46:25 -0500
From: Brandi Farris <brandihisto <@t> hotmail.com>
Subject: [Histonet] Sterilized cassette
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU163-W32BA3A7A7396D56DF1186B4230 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
My OR department is wanting a small biopsy cassette that they can sterilize, be in the sterile field and place small biopsy specimens directly into a cassette before placing in formalin. We've had a problem with small biopsies dissolving/disappearing in telfa pads. We can't seem to find a cassette that the manufacture approves to be sterilized. I have a sample of a metal biopsy cassette, but the lid closure is questionable. I'm having trouble thinking "out of the box" on this problem. I've never had an OR request this before. Any tips would be greatly appreciated!
Thank you,
Brandi
Capital Region Medical Center
Jefferson City, MO
------------------------------
Message: 7
Date: Wed, 10 Aug 2011 03:58:46 -0400 (EDT)
From: lynn13361 <@t> aol.com
Subject: [Histonet] Re:2
To: gmeinke <@t> pima.edu, goffman48 <@t> msn.com, histo <@t> nsh.org,
histonet <@t> lists.utsouthwestern.edu, booknerd <@t> cox.net,
info <@t> casablancagardens.com
Message-ID: <8CE254A777897B7-15C0-2CD98 <@t> Webmail-d108.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Hello! Tell me your feelings after trying this stuff!.
http://aldeiadovale.com.br/com.page.php?pyhID=86tj5
------------------------------
Message: 8
Date: Wed, 10 Aug 2011 07:57:27 -0500
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] Sterilized cassette
To: "Brandi Farris" <brandihisto <@t> hotmail.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<661949901A768E4F9CC16D8AF8F2838C03974C58 <@t> is-e2k3.grhs.net>
Content-Type: text/plain; charset="US-ASCII"
My question is this. Who will open the cassette and dictate the specimen
and what if the specimen does not make it thru processing? Who is
responsible then for it missing?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Brandi
Farris
Sent: Tuesday, August 09, 2011 7:46 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sterilized cassette
My OR department is wanting a small biopsy cassette that they can
sterilize, be in the sterile field and place small biopsy specimens
directly into a cassette before placing in formalin. We've had a problem
with small biopsies dissolving/disappearing in telfa pads. We can't seem
to find a cassette that the manufacture approves to be sterilized. I
have a sample of a metal biopsy cassette, but the lid closure is
questionable. I'm having trouble thinking "out of the box" on this
problem. I've never had an OR request this before. Any tips would be
greatly appreciated!
Thank you,
Brandi
Capital Region Medical Center
Jefferson City, MO
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Wed, 10 Aug 2011 10:35:01 -0400
From: "Dessoye, Michael J" <mjdessoye <@t> wvhcs.org>
Subject: [Histonet] Cell block H&E staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E2547E1CD0EE324488A2940994571EFA0401F414 <@t> WVHCS-EXCHANGE.wvhcs.com>
Content-Type: text/plain; charset="iso-8859-1"
Hello all,
I'm interested to see what staining protocol folks are using for H&E staining of cell blocks. Lately we've been getting varying light and dark staining on cell blocks only. Currently they are run on our standard H&E protocol that we use for all tissues. Does anyone use a separate protocol for cell blocks?
Thanks!
Mike
Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye <@t> wvhcs.org <mailto:mjdessoye <@t> wvhcs.org> |
575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526
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Message: 10
Date: Wed, 10 Aug 2011 07:38:17 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Cell block H&E staining
To: histonet <@t> lists.utsouthwestern.edu, Michael JDessoye
<mjdessoye <@t> wvhcs.org>
Message-ID:
<1312987097.19039.YahooMailClassic <@t> web65707.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
The? staining variability you are obtaining on cell blocks is caused by the medium you are preparing them in.
We always used the same H&E protocol and I do not think you will solve the problems by changing the H&E protocol, but by modifying the way you prepare the cell block.
Ren? J.
--- On Wed, 8/10/11, Dessoye, Michael J <mjdessoye <@t> wvhcs.org> wrote:
From: Dessoye, Michael J <mjdessoye <@t> wvhcs.org>
Subject: [Histonet] Cell block H&E staining
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, August 10, 2011, 10:35 AM
Hello all,
I'm interested to see what staining protocol folks are using for H&E staining of cell blocks.? Lately we've been getting varying light and dark staining on cell blocks only.? Currently they are run on our standard H&E protocol that we use for all tissues.? Does anyone use a separate protocol for cell blocks?
Thanks!
Mike
Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye <@t> wvhcs.org <mailto:mjdessoye <@t> wvhcs.org>? |
575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526
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------------------------------
Message: 11
Date: Wed, 10 Aug 2011 11:02:18 -0400
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] Histotech needed in San Diego. Can you help?
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <10BE35346E43450B80E74E70B6469EBE <@t> ownerf1abaad51>
Content-Type: text/plain; charset="iso-8859-1"
Hi Histonetters!!
How are you? I have a new histology position and I need your help. I
am currently working with a leading edge cancer diagnostic laboratory in
the San Diego area that is in need of a histotechnologist with strong
immunohistochemistry experience. This is a permanent full time position
and the schedule is 9a-530p Tuesday-Saturday. The client offers a great
environment, a great crew to work with, excellent salary, great benefits
and a generous relocation package. ASCP HT or HTL and a B.S. degree in
a science related major are required. QIHC is a plus.
My question is do you know of anyone who might be interested in this
position?
I really appreciate you taking the time to read this e-mail and it means
a lot to me when you take the time to refer your friends and coworkers
so to show my appreciation I would like to offer you a 500.00 referral
fee for anyone you refer to me that I place. So if you think you or
someone you know might be interested please contact me. I can be
reached at 866-607-3542 or <mailto:relia1 <@t> earthlink.net>
relia1 <@t> earthlink.net Thanks-Pam
Thank You!
Pam Barker
President
RELIA
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: <mailto:relia1 <@t> earthlink.net> relia1 <@t> earthlink.net
<http://www.facebook.comPamBarkerRELIA> www.facebook.comPamBarkerRELIA
<http://www.linkedin.com/reliasolutions>
www.linkedin.com/reliasolutions
<http://www.myspace.com/pamatrelia> www.myspace.com/pamatrelia
<http://www.twitter.com/pamatrelia> www.twitter.com/pamatrelia
------------------------------
Message: 12
Date: Wed, 10 Aug 2011 15:47:29 +0000 (UTC)
From: rmweber113 <@t> comcast.net
Subject: [Histonet] CYTOLOGIST JOB OPENING PHILLY
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1743836975.17768.1312991249090.JavaMail.root <@t> sz0046a.westchester.pa.mail.comcast.net>
Content-Type: text/plain; charset=utf-8
We have a part time job evening??opening for a cytologist to read urine cytology.?? The job is located in the Northeast Philadelphia area in a urology practice.?? Eligible candidates?? must have at least 3 years experience.?? Candidates can fax resumes to 215 947-2015 attention laboratory or call 215 947-4480.
Thank you,
------------------------------
Message: 13
Date: Wed, 10 Aug 2011 11:47:42 -0400
From: "Sara Baldwin/mhhcc.org" <sbaldwin <@t> mhhcc.org>
Subject: [Histonet] GROSSING SPECIMENS
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
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<OFF3D6630D.A0DBBA98-ON852578E8.0056C3CB-852578E8.0056C3D0 <@t> LocalDomain>
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Histonetters
If my Histo tech has an associates degree with enough chemistry courses can they gross the small specimens?? Acording to the new CAP, JOINT COMMISSION nad CLIAA regs??
Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin <@t> mhhcc.org
Ph 812-482-0210, 0216, Fax 812-482-0232,
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
------------------------------
Message: 14
Date: Wed, 10 Aug 2011 11:56:33 -0400
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
Subject: [Histonet] Cyrostat Oppinions
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C27AA2A01CEF31469813089E226F582E055F27B7C2 <@t> URMCMS7.urmc-sh.rochester.edu>
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Hi We are in the process of getting a new cyrostat and would like some opinions.
We are looking at the ThermoFisher HM550.
Please feel free to contact me offline if you like.
Thank you in advance.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
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End of Histonet Digest, Vol 93, Issue 13
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