[Histonet] thick sections and thaw freeze OCT tissues

mesruh turkekul turkekul <@t> gmail.com
Thu Aug 11 12:34:22 CDT 2011

I cut 40-50 micron paraffin sections with no difficulty. You may try to use
softer paraffin (lower melting temp) or adjust the blade angel. It seems
like you have chatter due to hardness of the block or not appropriate blade
angel. If tissue is over fixed or overprosessed and hence harder that it
should be the thicker you cut the more difficult it will be.

Mesruh Turkekul
turkekum <@t> mskcc.org

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