[Histonet] Cell block H&E staining

[Rene J Buesa]

The  staining variability you are obtaining on cell blocks is caused by the medium you are preparing them in.
We always used the same H&E protocol and I do not think you will solve the problems by changing the H&E protocol, but by modifying the way you prepare the cell block.
René J.

--- On Wed, 8/10/11, Dessoye, Michael J <mjdessoye <@t> wvhcs.org> wrote:


From: Dessoye, Michael J <mjdessoye <@t> wvhcs.org>
Subject: [Histonet] Cell block H&E staining
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, August 10, 2011, 10:35 AM


Hello all,

I'm interested to see what staining protocol folks are using for H&E staining of cell blocks.  Lately we've been getting varying light and dark staining on cell blocks only.  Currently they are run on our standard H&E protocol that we use for all tissues.  Does anyone use a separate protocol for cell blocks?

Thanks!
Mike

Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye <@t> wvhcs.org <mailto:mjdessoye <@t> wvhcs.org>  |
575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 
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