[Histonet] Reorienting frozen tissue

[Kim Merriam]

Whenever I have to reembed an OCT block, I leave it out at RT for just a couple of minutes to let it that a little bit.  Just take a razor blade and cut out the tissue (leaving some OCT around it); re-oreint it in a mold with RT OCT and then freeze it.  Don't freeze it too fast, you want the old OCT to melt a little bit with the new OCT, that way you will have a block without any seams in it.
 
Good luck!
Kim

Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA

From: Daniela Bodemer <daniela.bodemer <@t> mcri.edu.au>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tuesday, August 2, 2011 7:44 PM
Subject: [Histonet] Reorienting frozen tissue

Hi all,



I would like to know if it is possible and how to reorient or reembedd
human tissue, that has been frozen at -80C in OCT. 



Many thanks,



Daniela Bodemer 

Research Assistant

Surgical Research, Infection and Immunity



Murdoch Childrens Research Institute

The Royal Children's Hospital

Flemington Road Parkville Victoria 3052 Australia 

T 03 9345 5930    T (03 9345 4116)    

E daniela.bodemer <@t> mcri.edu.au <mailto:firstname.surname <@t> mcri.edu.au> 

www.mcri.edu.au <http://www.mcri.edu.au/> 



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