[Histonet] Re: Histonet Digest, Vol 89, Issue 14
Poupak Farahani
poupakfar <@t> yahoo.com
Sat Apr 30 18:00:07 CDT 2011
Evimjuytdr e
Sent from my iPad
On Apr 18, 2011, at 7:43 AM, histonet-request <@t> lists.utsouthwestern.edu wrote:
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request <@t> lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. Renal Catecholamines (Lynne Cates)juuuhggswewfqgb verydffgffhfshopqaDDHWYDCFGHK
> 2. RE: Ventana ultra (Kim Tournear)
> 3. Re: Ventana ultra (Angela Bitting)
> 4. AW: [Histonet] Benchmark Ultra question - follow up (Gudrun Lang)
> 5. RE: Ventana ultra (Kuhnla, Melissa)
> 6. Re: FW: 1 H&E slide vs. 2 (Rene J Buesa)
> 7. RE: Ventana ultra (Amber McKenzie)
> 8. RE: Ventana ultra (Setlak, Lisa)
> 9. AW: [Histonet] Ventana ultra (Gudrun Lang)
> 10. RE: Histonet Digest, Von Kossa Stain (Mayer,Toysha N)
> 11. IHC platforms/Sales Representatives (Paula Lucas)
> 12. Re: AW: [Histonet] Benchmark Ultra question - follow up
> (Angela Bitting)
> 13. RE: Ventana ultra (Angela Bitting)
> 14. slide brite and coverslip drying time (Dawn Herron)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 13 Apr 2011 10:13:23 -0400
> From: "Lynne Cates" <lcates <@t> synecor.com>
> Subject: [Histonet] Renal Catecholamines
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <A0364DF9F7B03A499C5BE6D3FB343E60018FC75D <@t> synecor-main.CORP.synecor.local>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anyone know any places that perform catecholamines on porcine renal
> tissue and contact numbers?
>
>
>
> Lynne
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 13 Apr 2011 07:32:56 -0700 (PDT)
> From: Kim Tournear <kim.tournear <@t> yahoo.com>
> Subject: RE: [Histonet] Ventana ultra
> To: Barbara.Crill <@t> LPNT.net, histonet <@t> lists.utsouthwestern.edu, Sebree
> Linda A <LSebree <@t> uwhealth.org>
> Message-ID: <504386.23697.qm <@t> web120220.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> I've heard that adding slides and/or reagents can add as much as 8 minutes to the run time. Again, that's just what I've heard, don't know if it's fact or fiction....
>
> ~Kim~
> OU ROCKS!!!!
> ~Don't be afraid your life will end,
> be afraid it will never begin~
>
> --- On Wed, 4/13/11, Sebree Linda A <LSebree <@t> uwhealth.org> wrote:
>
>
> From: Sebree Linda A <LSebree <@t> uwhealth.org>
> Subject: RE: [Histonet] Ventana ultra
> To: Barbara.Crill <@t> LPNT.net, histonet <@t> lists.utsouthwestern.edu
> Date: Wednesday, April 13, 2011, 2:09 PM
>
>
> Please "Reply All" as I'd like to hear feedback also.
>
> Thanks.
>
>
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> DB1-223 VAH
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Barbara.Crill <@t> LPNT.net
> Sent: Wednesday, April 13, 2011 8:39 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Ventana ultra
>
> Does anyone use the Benchmark Ultra - care to share your comments?
>
> We are considering switching from the Benchmark XT to the Ultra but
> would like to hear from users about this instrument.
> Is there an increase in reagent cost?
>
> Can you really add more slides without adding time to the run?
>
>
> ANTOINETTE CRILL, MBA,CT(ASCP)
> TEAM LEADER ANATOMIC PATHOLOGY
> DANVILLE REGIONAL MEDICAL CENTER
> (O) 434.799.4470
> (F) 434.773.6806
> E-mail: barbara.crill <@t> LPNT.net<mailto:barbara.crill <@t> LPNT.net>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Wed, 13 Apr 2011 10:41:42 -0400
> From: "Angela Bitting" <akbitting <@t> geisinger.edu>
> Subject: Re: [Histonet] Ventana ultra
> To: <histonet <@t> lists.utsouthwestern.edu>,<Barbara.Crill <@t> LPNT.net>
> Message-ID: <4DA57DE6.2B7F.00C9.1 <@t> geisinger.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> each of the 30 drawers essentially is it's own "run". You don't have to wait for all 30 drawers to finish their runs before adding more slides.
> There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT.
> The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability.
>
>
> Angela Bitting, HT(ASCP), QIHC
> Technical Specialist, Histology
> Geisinger Medical Center
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone 570-214-9634
> fax 570-271-5916
>
> No trees were hurt in the sending of this email
> However many electrons were severly inconvienienced!
>
>>>> <Barbara.Crill <@t> LPNT.net> 4/13/2011 9:39 AM >>>
> Does anyone use the Benchmark Ultra - care to share your comments?
>
> We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument.
> Is there an increase in reagent cost?
>
> Can you really add more slides without adding time to the run?
>
>
> ANTOINETTE CRILL, MBA,CT(ASCP)
> TEAM LEADER ANATOMIC PATHOLOGY
> DANVILLE REGIONAL MEDICAL CENTER
> (O) 434.799.4470
> (F) 434.773.6806
> E-mail: barbara.crill <@t> LPNT.net<mailto:barbara.crill <@t> LPNT.net>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
> -------------- next part --------------
> BEGIN:VCARD
> VERSION:2.1
> X-GWTYPE:USER
> FN:Bitting, Angela
> TEL;WORK:570-271-6844
> ORG:;Histology
> EMAIL;WORK;PREF;NGW:AKBITTING <@t> geisinger.edu
> N:Bitting;Angela
> END:VCARD
>
>
> ------------------------------
>
> Message: 4
> Date: Wed, 13 Apr 2011 16:44:27 +0200
> From: "Gudrun Lang" <gu.lang <@t> gmx.at>
> Subject: AW: [Histonet] Benchmark Ultra question - follow up
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <87A14A80DD074AA5A3308A1E3BDD40BA <@t> dielangs.at>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Thank you all for your replies. After all I have to admit, that the cause
> was human failure. We found, that the protocols have been changed and noone
> did remember. That was after a stopped run, where HIER war clicked off for
> the repetition. And afterward only short HIER was clicked on again instead
> of mild HIER. – stupid me!
>
>
>
> Bye Gudrun
>
>
>
> _____
>
> Von: Lynn Lee [mailto:lynnlee2010 <@t> live.com]
> Gesendet: Mittwoch, 13. April 2011 04:14
> An: gu.lang <@t> gmx.at
> Betreff: FW: [Histonet] Benchmark Ultra question - follow up
>
>
>
>
> One last thought- do you filter the new antibody solution you made EVERY
> time when refilling the prep kit? If not, there could be some particulates
> in the diluent causing a blockage also. Hope these suggestions help or
> maybe you have already checked this out.
>
> Lynn Lee
>
>
>
>
>
> _____
>
> From: lynnlee2010 <@t> live.com
> To: gu.lang <@t> gmx.at
> Subject: RE: [Histonet] Benchmark Ultra question - follow up
> Date: Tue, 12 Apr 2011 20:10:43 -0600
>
> It's possible the flip lid on the prep kit dispenser top is not completed
> closed and seated properly. When the dispense hammer goes down, the pressure
> may not be the same, every time if this is the case. I always check the tip
> of the dispenser for air bubbles before putting on the machine for EVERY one
> whether it is a prefilled Ventana dispenser or my prep kit dispenser.
> Finally something could be clogged in a line somewhere.
>
> I worked in R & D for Ventana for almost 4 years and have since used the XT
> and Ultra in other labs and never had a problem with incorrect amount of
> reagent being dispensed. I guess there's a first time for anything though. I
> would call Ventana Customer Support. They are great!
>
> Lynn Lee B.S., HT(ASCP)
> Tucson, AZ
>
>
>> From: gu.lang <@t> gmx.at
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Tue, 12 Apr 2011 18:23:27 +0200
>> Subject: [Histonet] Benchmark Ultra question - follow up
>>
>> Today I made a further experiment. I did two titration runs. On one slide
> I
>> added the working solution by pipette on the other slide I added it by
>> pushing the filled PrepKit.
>> Both stainings came out beautiful. - There has to be something wrong with
>> the dispension-amount. Ventana is informed, I hope they find the culprit.
>>
>> Bye
>> Gudrun
>>
>> -----Ursprüngliche Nachricht-----
>> Von: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Gudrun
>> Lang
>> Gesendet: Montag, 11. April 2011 18:48
>> An: 'Angela Bitting'
>> Cc: histonet <@t> lists.utsouthwestern.edu
>> Betreff: AW: [Histonet] Benchmark Ultra question
>>
>> Yes, that could be true. Some of the PrepKits "go harder" than others.
>> Perhaps the pressure of the hammer is not big enough. Sometimes the
> nozzles
>> are not filled equally. (We press always reagens in the nozzle when we
>> prepare the run.)
>>
>> With the Ventana-System there is an additional dilution, because the
>> working-solution is added to a reaction-buffer film under the LCS. Perhaps
>> it makes a difference if you dispense the solution with the pipett-tip
> under
>> the LCS or if the drop falls on the surface of the LCS. That could be a
>> matter of the LCS-quality, or buffer-quantity , or . I think I'll become
>> mad!
>>
>> Our pathologists have an uncertain unhappiness with the overall staining
>> results, but most of the antibodies work well. Perhaps the majority isn't
>> very sensitiv to small changes in the system and the quality-difference
>> isn't big enough, but some are easier to "kill".
>>
>> Regards, Gudrun
>>
>>
>>
>>
>>
>> _____
>>
>> Von: Angela Bitting [mailto:akbitting <@t> geisinger.edu]
>> Gesendet: Montag, 11. April 2011 18:06
>> An: gu.lang <@t> gmx.at
>> Betreff: Re: [Histonet] Benchmark Ultra question
>>
>>
>>
>> When I was trained to do titration runs on the BenchmarkXT and Ultras, I
> was
>> told to titrate 100ul of antibody. I have been suspecting for some time
> now
>> that the dispensers DON'T dispense a full 100ul. I haven't taken the time
> to
>> prove it, but your question may have motivated me.
>>
>> That could explain the weak staining.
>>
>>>>> "Gudrun Lang" <gu.lang <@t> gmx.at> 4/11/2011 11:48 AM >>>
>> Hi!
>>
>> I have some issues with our new Ultra. Since a few days antibodies, that
>> work usually well, show up very faint. For example the TTF1 (Novocastra).
>>
>> I ordered a new bottle, made a titration and found, that the "old" titer
>> 1:50 was again well enough. I filled the old PrepKit - and the result was
>> very weak.
>>
>> Then I thought, the PrepKit itself is the culprit. I changed the PrepKit -
>> and again the result was very weak. At that time the working-solution was
>> only three days old.
>>
>>
>>
>> Has anyone an explanation, why the titration works well and the automated
>> dispension doesn't?
>>
>>
>>
>> Regards
>>
>> Gudrun Lang
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>> _____
>>
>> IMPORTANT WARNING: The information in this message (and the documents
>> attached to it, if any) is confidential and may be legally privileged. It
> is
>> intended solely for the addressee. Access to this message by anyone else
> is
>> unauthorized. If you are not the intended recipient, any disclosure,
>> copying, distribution or any action taken, or omitted to be taken, in
>> reliance on it is prohibited and may be unlawful. If you have received
> this
>> message in error, please delete all electronic copies of this message (and
>> the documents attached to it, if any), destroy any hard copies you may
> have
>> created and notify me immediately by replying to this email. Thank you.
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 5
> Date: Wed, 13 Apr 2011 10:45:06 -0400
> From: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>
> Subject: RE: [Histonet] Ventana ultra
> To: <Barbara.Crill <@t> LPNT.net>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <C76F12086768614F9C2618ED9966FEE10698F427 <@t> MMDCNT0BXVS003.chsli.org>
> Content-Type: text/plain; charset="us-ascii"
>
> I currently have one XT and 3 Ultras. I love the Ultras. There is no
> difference in reagent costs. The only thing that I can say remotely
> related is that if you keep both platforms, the XT and the ultras have
> separate CC1, CC2, and LCS. I am fairly certain the contents of the
> bottle are the same, but the label and part numbers are different.
> I love the continuous access. I have it set up where the three ultras
> are 'top loaded' with set antibodies every day. This eliminates bouncing
> things around all the time. Contact me if you would like anymore info. I
> would be happy to help. I notice no delay in run times.
> melissa
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Barbara.Crill <@t> LPNT.net
> Sent: Wednesday, April 13, 2011 9:39 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Ventana ultra
>
> Does anyone use the Benchmark Ultra - care to share your comments?
>
> We are considering switching from the Benchmark XT to the Ultra but
> would like to hear from users about this instrument.
> Is there an increase in reagent cost?
>
> Can you really add more slides without adding time to the run?
>
>
> ANTOINETTE CRILL, MBA,CT(ASCP)
> TEAM LEADER ANATOMIC PATHOLOGY
> DANVILLE REGIONAL MEDICAL CENTER
> (O) 434.799.4470
> (F) 434.773.6806
> E-mail: barbara.crill <@t> LPNT.net<mailto:barbara.crill <@t> LPNT.net>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you.
>
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Wed, 13 Apr 2011 07:52:04 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] FW: 1 H&E slide vs. 2
> To: histonet <@t> lists.utsouthwestern.edu, Eugenia Thomas
> <eugenia902d1 <@t> hotmail.com>
> Message-ID: <748035.62239.qm <@t> web65706.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> There is not such a study. Cutting 2 vs. 1 H&E/block is merely a preference issue amongst pathologists. Now, if you refer to TWO different levels, that is a different issue.
> Two consecutive-serial sections from each block should not have a diagnostic impact BUT two different levels, separated by 20-25 sections, may.
> That is a decision to take by the pathologist.
> René J.
>
> --- On Tue, 4/12/11, Eugenia Thomas <eugenia902d1 <@t> hotmail.com> wrote:
>
>
> From: Eugenia Thomas <eugenia902d1 <@t> hotmail.com>
> Subject: [Histonet] FW: 1 H&E slide vs. 2
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tuesday, April 12, 2011, 11:14 PM
>
>
>
>
>
>
>
> From: eugenia902d1 <@t> hotmail.com
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: 1 H&E slide vs. 2
> Date: Mon, 11 Apr 2011 13:06:59 -0400
>
>
>
>
> Good afternoon everyone,
>
> Does anyone know of an article or statistics discussing the impact (medical diagnosing) of cutting 2 H&E slides per block verses 1 for all routine work?
>
>
> Genia
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Wed, 13 Apr 2011 09:55:38 -0500
> From: "Amber McKenzie" <amber.mckenzie <@t> gastrodocs.net>
> Subject: RE: [Histonet] Ventana ultra
> To: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>,
> <Barbara.Crill <@t> LPNT.net>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <03C921A1EAF7F541B16543F6EC6A4B370407E57C <@t> giamail2.Gia.com>
> Content-Type: text/plain; charset="us-ascii"
>
> I recently got an Ultra about a month ago and doing test runs, I have
> really enjoyed the continuous feed as well but I have a question...if I
> deactivate a product from the Ultra's inventory list I notice it's still
> in my XT's inventory list. So, I have to deactivate everything off of
> each instrument?
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kuhnla,
> Melissa
> Sent: Wednesday, April 13, 2011 9:45 AM
> To: Barbara.Crill <@t> LPNT.net; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Ventana ultra
>
> I currently have one XT and 3 Ultras. I love the Ultras. There is no
> difference in reagent costs. The only thing that I can say remotely
> related is that if you keep both platforms, the XT and the ultras have
> separate CC1, CC2, and LCS. I am fairly certain the contents of the
> bottle are the same, but the label and part numbers are different.
> I love the continuous access. I have it set up where the three ultras
> are 'top loaded' with set antibodies every day. This eliminates bouncing
> things around all the time. Contact me if you would like anymore info. I
> would be happy to help. I notice no delay in run times.
> melissa
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Barbara.Crill <@t> LPNT.net
> Sent: Wednesday, April 13, 2011 9:39 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Ventana ultra
>
> Does anyone use the Benchmark Ultra - care to share your comments?
>
> We are considering switching from the Benchmark XT to the Ultra but
> would like to hear from users about this instrument.
> Is there an increase in reagent cost?
>
> Can you really add more slides without adding time to the run?
>
>
> ANTOINETTE CRILL, MBA,CT(ASCP)
> TEAM LEADER ANATOMIC PATHOLOGY
> DANVILLE REGIONAL MEDICAL CENTER
> (O) 434.799.4470
> (F) 434.773.6806
> E-mail: barbara.crill <@t> LPNT.net<mailto:barbara.crill <@t> LPNT.net>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> The information in this e-mail, and any attachments therein, is
> confidential and for use by the intended addressee only. If this message
> is received by you in error please do not disseminate or read further.
> Please reply to the sender that you have received the message in error,
> then delete the message. Although Catholic Health Services of Long
> Island attempts to sweep e-mail and attachments for viruses, it does
> not guarantee that either are virus-free and accepts no liability for
> any damage sustained as a result of viruses. Thank you.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 8
> Date: Wed, 13 Apr 2011 09:54:44 -0500
> From: "Setlak, Lisa" <LSetlak <@t> childrensmemorial.org>
> Subject: RE: [Histonet] Ventana ultra
> To: 'Angela Bitting' <akbitting <@t> geisinger.edu>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, "Barbara.Crill <@t> LPNT.net"
> <Barbara.Crill <@t> LPNT.net>
> Message-ID:
> <7111DB39D045004C9CF29E79C71B28BC101D507915 <@t> CMHEXCC01MBX.childrensmemorial.org>
>
> Content-Type: text/plain; charset="us-ascii"
>
> We like ours as well. It's not as continual as we originally thought.... to maximize its potential I would recommend placing frequently used antibodies on the wheel with the first run. As long as you have the antibody you want to stain for on the wheel you can add the slide anytime you want and it will run it. If the antibody is not on the wheel you have to set a "landing zone" in order to add it. This usually works out fine, however once the run that is already going reaches a certain point, you aren't able to set a landing zone and may have to wait awhile to load new slides. It is pretty cool that you can run FITC, FISH, and IHC at the same time (however we tend to put our EBER on overnight because it's a fairly long run).
> Lisa
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Angela Bitting
> Sent: Wednesday, April 13, 2011 9:42 AM
> To: histonet <@t> lists.utsouthwestern.edu; Barbara.Crill <@t> LPNT.net
> Subject: Re: [Histonet] Ventana ultra
>
> each of the 30 drawers essentially is it's own "run". You don't have to wait for all 30 drawers to finish their runs before adding more slides.
> There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT.
> The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability.
>
>
> Angela Bitting, HT(ASCP), QIHC
> Technical Specialist, Histology
> Geisinger Medical Center
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone 570-214-9634
> fax 570-271-5916
>
> No trees were hurt in the sending of this email
> However many electrons were severly inconvienienced!
>
>>>> <Barbara.Crill <@t> LPNT.net> 4/13/2011 9:39 AM >>>
> Does anyone use the Benchmark Ultra - care to share your comments?
>
> We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument.
> Is there an increase in reagent cost?
>
> Can you really add more slides without adding time to the run?
>
>
> ANTOINETTE CRILL, MBA,CT(ASCP)
> TEAM LEADER ANATOMIC PATHOLOGY
> DANVILLE REGIONAL MEDICAL CENTER
> (O) 434.799.4470
> (F) 434.773.6806
> E-mail: barbara.crill <@t> LPNT.net<mailto:barbara.crill <@t> LPNT.net>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
>
>
>
> ------------------------------
>
> Message: 9
> Date: Wed, 13 Apr 2011 17:01:35 +0200
> From: "Gudrun Lang" <gu.lang <@t> gmx.at>
> Subject: AW: [Histonet] Ventana ultra
> To: <Barbara.Crill <@t> LPNT.net>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <49E6A03ED68944AEA42E8F4F115F9012 <@t> dielangs.at>
> Content-Type: text/plain; charset="iso-8859-1"
>
> What we like about the Ultra is, that there's no need for cleaning runs
> between the runs and that you can take out the bulk and waste containers
> during the run.
> We don't use the continious loading, because it doesn't fit to our workplan
> and because we use various antibodies from run to run.
> The runs are faster, but more than 2 runs per 8 hour shift and 1 run over
> night are not possible for us. That's the same workload we did with the XT.
>
> There are "windows" during the run, when you can add new slides and reagens.
> If the needed reagens in on the instrument, you can add them any time, if
> there's enough place. But the run will last longer and you have to wait for
> a "window" of the longer run to get the reagens off the instrument. Ready
> stained slides can be taken out independently. But the free pads cannot be
> filled and runs started, until you have the possibility to add the right
> reagens. So we decided to stay with "patch-workload" and real go-away.
>
> Bye
> Gudrun
>
> -----Ursprüngliche Nachricht-----
> Von: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von
> Barbara.Crill <@t> LPNT.net
> Gesendet: Mittwoch, 13. April 2011 15:39
> An: histonet <@t> lists.utsouthwestern.edu
> Betreff: [Histonet] Ventana ultra
>
> Does anyone use the Benchmark Ultra - care to share your comments?
>
> We are considering switching from the Benchmark XT to the Ultra but would
> like to hear from users about this instrument.
> Is there an increase in reagent cost?
>
> Can you really add more slides without adding time to the run?
>
>
> ANTOINETTE CRILL, MBA,CT(ASCP)
> TEAM LEADER ANATOMIC PATHOLOGY
> DANVILLE REGIONAL MEDICAL CENTER
> (O) 434.799.4470
> (F) 434.773.6806
> E-mail: barbara.crill <@t> LPNT.net<mailto:barbara.crill <@t> LPNT.net>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Wed, 13 Apr 2011 10:12:18 -0500
> From: "Mayer,Toysha N" <TNMayer <@t> mdanderson.org>
> Subject: [Histonet] RE: Histonet Digest, Von Kossa Stain
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <DFD2C49464F83A4F9201E2D07100774E193F4DEE1A <@t> DCPWVMBXC0VS3.mdanderson.edu>
>
> Content-Type: text/plain; charset="us-ascii"
>
> We used the lamp at our cutting stations, coplin jar in our waterbath or on top of the embedder, wrapped in tin foil for 1 hr.
>
> Toysha N. Mayer, MBA, HT (ASCP)
> Education Coordinator
> Program in Histotechnology
> School of Health Professions
> MD Anderson Cancer Center
> (713) 563-3481
> tnmayer <@t> mdanderson.org
>
>
>
>
> What is everyone using for their "light" when developing the silver in the VonKossa stain when you have no sunlight to use? We used to use a 60 watt lamp, but haven't done one for years and am bringing this stain back to our repetiore due to pathologist request. Thanks much!
>
> Dorothy Webb, HT (ASCP)
> Regions Histology Technical Supervisor
> 651-254-2962
>
>
>
>
>
>
>
>
>
>
>
>
> ------------------------------
>
>
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Wed, 13 Apr 2011 08:29:39 -0700
> From: "Paula Lucas" <plucas <@t> biopath.org>
> Subject: [Histonet] IHC platforms/Sales Representatives
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <6619BD7E2C004B4FA2E53529F39EB9B1 <@t> biopath.local>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello,
>
>
>
> We are inquiring about IHC staining platforms and we've already contacted
> the Leica sales rep for the Bond. I would also like to contact Ventana to
> get a quote on their system, and I've tried to get in contact with a sales
> rep in our area but I haven't had anyone returning my calls. If there is a
> Vantana rep reading this, would you please contact the sales representative
> for my area and let him/her know we are interested in a quote? We are
> located in Orange County (Fountain Valley), California.
>
>
>
> For those who have experience with these two platforms, would you mind
> taking the time to share with me your views and experiences about the two
> systems?
>
>
>
> Thanks so much,
>
> Paula Lucas
>
> Lab Manager
>
> Bio-Path Medical Group
>
> Fountain Valley, CA
>
> 714.433.1330
>
>
>
> ------------------------------
>
> Message: 12
> Date: Wed, 13 Apr 2011 11:30:43 -0400
> From: "Angela Bitting" <akbitting <@t> geisinger.edu>
> Subject: Re: AW: [Histonet] Benchmark Ultra question - follow up
> To: <gu.lang <@t> gmx.at>,<histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <4DA58963.2B7F.00C9.1 <@t> geisinger.edu>
> Content-Type: text/plain; charset=UTF-8
>
> Augh! I hate when that happens!
>
>>>> "Gudrun Lang" <gu.lang <@t> gmx.at> 4/13/2011 10:44 AM >>>
> Thank you all for your replies. After all I have to admit, that the
> cause
> was human failure. We found, that the protocols have been changed and
> noone
> did remember. That was after a stopped run, where HIER war clicked off
> for
> the repetition. And afterward only short HIER was clicked on again
> instead
> of mild HIER. – stupid me!
>
>
>
> Bye Gudrun
>
>
>
> _____
>
> Von: Lynn Lee [mailto:lynnlee2010 <@t> live.com]
> Gesendet: Mittwoch, 13. April 2011 04:14
> An: gu.lang <@t> gmx.at
> Betreff: FW: [Histonet] Benchmark Ultra question - follow up
>
>
>
>
> One last thought- do you filter the new antibody solution you made
> EVERY
> time when refilling the prep kit? If not, there could be some
> particulates
> in the diluent causing a blockage also. Hope these suggestions help
> or
> maybe you have already checked this out.
>
> Lynn Lee
>
>
>
>
>
> _____
>
> From: lynnlee2010 <@t> live.com
> To: gu.lang <@t> gmx.at
> Subject: RE: [Histonet] Benchmark Ultra question - follow up
> Date: Tue, 12 Apr 2011 20:10:43 -0600
>
> It's possible the flip lid on the prep kit dispenser top is not
> completed
> closed and seated properly. When the dispense hammer goes down, the
> pressure
> may not be the same, every time if this is the case. I always check
> the tip
> of the dispenser for air bubbles before putting on the machine for
> EVERY one
> whether it is a prefilled Ventana dispenser or my prep kit dispenser.
> Finally something could be clogged in a line somewhere.
>
> I worked in R & D for Ventana for almost 4 years and have since used
> the XT
> and Ultra in other labs and never had a problem with incorrect amount
> of
> reagent being dispensed. I guess there's a first time for anything
> though. I
> would call Ventana Customer Support. They are great!
>
> Lynn Lee B.S., HT(ASCP)
> Tucson, AZ
>
>
>> From: gu.lang <@t> gmx.at
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Tue, 12 Apr 2011 18:23:27 +0200
>> Subject: [Histonet] Benchmark Ultra question - follow up
>>
>> Today I made a further experiment. I did two titration runs. On one
> slide
> I
>> added the working solution by pipette on the other slide I added it
> by
>> pushing the filled PrepKit.
>> Both stainings came out beautiful. - There has to be something wrong
> with
>> the dispension-amount. Ventana is informed, I hope they find the
> culprit.
>>
>> Bye
>> Gudrun
>>
>> -----Ursprüngliche Nachricht-----
>> Von: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von
> Gudrun
>> Lang
>> Gesendet: Montag, 11. April 2011 18:48
>> An: 'Angela Bitting'
>> Cc: histonet <@t> lists.utsouthwestern.edu
>> Betreff: AW: [Histonet] Benchmark Ultra question
>>
>> Yes, that could be true. Some of the PrepKits "go harder" than
> others.
>> Perhaps the pressure of the hammer is not big enough. Sometimes the
> nozzles
>> are not filled equally. (We press always reagens in the nozzle when
> we
>> prepare the run.)
>>
>> With the Ventana-System there is an additional dilution, because the
>> working-solution is added to a reaction-buffer film under the LCS.
> Perhaps
>> it makes a difference if you dispense the solution with the
> pipett-tip
> under
>> the LCS or if the drop falls on the surface of the LCS. That could be
> a
>> matter of the LCS-quality, or buffer-quantity , or . I think I'll
> become
>> mad!
>>
>> Our pathologists have an uncertain unhappiness with the overall
> staining
>> results, but most of the antibodies work well. Perhaps the majority
> isn't
>> very sensitiv to small changes in the system and the
> quality-difference
>> isn't big enough, but some are easier to "kill".
>>
>> Regards, Gudrun
>>
>>
>>
>>
>>
>> _____
>>
>> Von: Angela Bitting [mailto:akbitting <@t> geisinger.edu]
>> Gesendet: Montag, 11. April 2011 18:06
>> An: gu.lang <@t> gmx.at
>> Betreff: Re: [Histonet] Benchmark Ultra question
>>
>>
>>
>> When I was trained to do titration runs on the BenchmarkXT and
> Ultras, I
> was
>> told to titrate 100ul of antibody. I have been suspecting for some
> time
> now
>> that the dispensers DON'T dispense a full 100ul. I haven't taken the
> time
> to
>> prove it, but your question may have motivated me.
>>
>> That could explain the weak staining.
>>
>>>>> "Gudrun Lang" <gu.lang <@t> gmx.at> 4/11/2011 11:48 AM >>>
>> Hi!
>>
>> I have some issues with our new Ultra. Since a few days antibodies,
> that
>> work usually well, show up very faint. For example the TTF1
> (Novocastra).
>>
>> I ordered a new bottle, made a titration and found, that the "old"
> titer
>> 1:50 was again well enough. I filled the old PrepKit - and the result
> was
>> very weak.
>>
>> Then I thought, the PrepKit itself is the culprit. I changed the
> PrepKit -
>> and again the result was very weak. At that time the working-solution
> was
>> only three days old.
>>
>>
>>
>> Has anyone an explanation, why the titration works well and the
> automated
>> dispension doesn't?
>>
>>
>>
>> Regards
>>
>> Gudrun Lang
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>> _____
>>
>> IMPORTANT WARNING: The information in this message (and the
> documents
>> attached to it, if any) is confidential and may be legally
> privileged. It
> is
>> intended solely for the addressee. Access to this message by anyone
> else
> is
>> unauthorized. If you are not the intended recipient, any disclosure,
>> copying, distribution or any action taken, or omitted to be taken,
> in
>> reliance on it is prohibited and may be unlawful. If you have
> received
> this
>> message in error, please delete all electronic copies of this message
> (and
>> the documents attached to it, if any), destroy any hard copies you
> may
> have
>> created and notify me immediately by replying to this email. Thank
> you.
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 13
> Date: Wed, 13 Apr 2011 11:36:20 -0400
> From: "Angela Bitting" <akbitting <@t> geisinger.edu>
> Subject: RE: [Histonet] Ventana ultra
> To: "Lisa Setlak" <LSetlak <@t> childrensmemorial.org>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, "Barbara.Crill <@t> LPNT.net"
> <Barbara.Crill <@t> LPNT.net>
> Message-ID: <4DA58AB4.2B7F.00C9.1 <@t> geisinger.edu>
> Content-Type: text/plain; charset=US-ASCII
>
> We load our Ultras with the most commonly used antibodies each morning. So that minimizes the number of landing zones we need to use.
> Also, (if you run both XTs and Ultras) run your antibodies with the longest protocols on Ultra and that will shorten your XT runs.
>
>>>> "Setlak, Lisa" <LSetlak <@t> childrensmemorial.org> 4/13/2011 10:54 AM >>>
> We like ours as well. It's not as continual as we originally thought.... to maximize its potential I would recommend placing frequently used antibodies on the wheel with the first run. As long as you have the antibody you want to stain for on the wheel you can add the slide anytime you want and it will run it. If the antibody is not on the wheel you have to set a "landing zone" in order to add it. This usually works out fine, however once the run that is already going reaches a certain point, you aren't able to set a landing zone and may have to wait awhile to load new slides. It is pretty cool that you can run FITC, FISH, and IHC at the same time (however we tend to put our EBER on overnight because it's a fairly long run).
> Lisa
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Angela Bitting
> Sent: Wednesday, April 13, 2011 9:42 AM
> To: histonet <@t> lists.utsouthwestern.edu; Barbara.Crill <@t> LPNT.net
> Subject: Re: [Histonet] Ventana ultra
>
> each of the 30 drawers essentially is it's own "run". You don't have to wait for all 30 drawers to finish their runs before adding more slides.
> There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT.
> The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability.
>
>
> Angela Bitting, HT(ASCP), QIHC
> Technical Specialist, Histology
> Geisinger Medical Center
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone 570-214-9634
> fax 570-271-5916
>
> No trees were hurt in the sending of this email
> However many electrons were severly inconvienienced!
>
>>>> <Barbara.Crill <@t> LPNT.net> 4/13/2011 9:39 AM >>>
> Does anyone use the Benchmark Ultra - care to share your comments?
>
> We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument.
> Is there an increase in reagent cost?
>
> Can you really add more slides without adding time to the run?
>
>
> ANTOINETTE CRILL, MBA,CT(ASCP)
> TEAM LEADER ANATOMIC PATHOLOGY
> DANVILLE REGIONAL MEDICAL CENTER
> (O) 434.799.4470
> (F) 434.773.6806
> E-mail: barbara.crill <@t> LPNT.net<mailto:barbara.crill <@t> LPNT.net>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
>
>
>
>
> -------------------------------------------------------------------------
> This message was secured by ZixCorp(R).
>
>
> ------------------------------
>
> Message: 14
> Date: Wed, 13 Apr 2011 11:16:48 -0500
> From: Dawn Herron <dwenzel01 <@t> gmail.com>
> Subject: [Histonet] slide brite and coverslip drying time
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <BANLkTikrTnpr9Xd73TkcXa5RyJHhmAfFrw <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hello all. We have just made the transition from xylene in our linear
> stainer to Slide Brite. We are also coverslipping from Slide Brite with
> Permount. So far the slides look good (though we have to be careful about
> water contamination in the dehydrating alcohols) but we are having a problem
> with extremely long coverslipping drying time. Some of the slides have been
> on the warmer for over 18 hours and you can still move the coverslips!
> (Normally the slides would dry within 4 hours on the warmer with xylene and
> acrymount) Any suggestions on how to speed drying time?
>
> Thanks,
> Dawn
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 89, Issue 14
> ****************************************
More information about the Histonet
mailing list