[Histonet] cap new ANP.22760

[Settembre, Dana]

ANTIBODY LOTS
I have a Dako and I have a Bond too.
For the Dako I use my positive control. I use 3 slides.
One is treated as the negative control - no antibody
One is treated with the current lot 
One is treated with the new lot.
I keep all QC slides together.
I keep the 3 slides together.

For my Bond,
I use 2 slides of my positive control
When a new lot comes in I run the new lot and the negative control.
Then I compare them to the previous lot that I ran in the past.
I keep the slides in chronological order - the previous lot goes back with
The slides from that date. The NEW Lot slides get filed with today's date.

DETECTION SYSTEM LOTS
For the Bond I QC the detection kit using an antibody like Ki-67, it works well and always shows good nuclear staining.
I indicate the received date, and QC date.
I run the QC on my Ki-67 positive control.  I use 2 slides.
One is treated as the negative control - no antibody.
One is treated with Ki-67.
I compare these to the previous Detection kit QC slides

I created a written procedures for all of the above.

Hope this helps.

Dana Settembre
Immunohistochemistry Lab
University Hospital - UMDNJ
Newark, NJ

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of anita dudley
Sent: Friday, April 29, 2011 12:30 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cap new ANP.22760


hi everyone,  just wondering how others were handling this checklist statement?  thanks so much for your input.  seems 
like a lot of extra work when running control with each show if each is working.
 
anita dudley
providence hosp
mobile alabama 		 	   		  _______________________________________________
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