[Histonet] Tumors tumors everywhere

[Akemi Allison]

Sarah,
I am curious; have you tried contacting the BioCare research tech  
support reps?  They are very helpful regarding their products and  
trouble-shooting any issues that may arise.


Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3377 <@t> yahoo.com

On Apr 28, 2011, at 1:16 PM, <sgoebel <@t> mirnarx.com>  
<sgoebel <@t> mirnarx.com> wrote:

> So I am staining tumors that were implanted as cells subdermally into
> mice.  The cells are human.  I am trying to do Caspase staining on  
> these
> tumors.  The primary is an anti-human rabbit polyclonal, and I am  
> using
> a polymer (Biocare Mach3) in lieu of the secondary antibody.  The
> background is through the roof!!  Could the reason be that the  
> tumor was
> grown in a mouse and is having cross reactivity somehow?  What species
> antibody should I be using instead?  All my mouse monoclonal  
> antibodies
> work perfect on the tissue, it's this stupid rabbit polyclonal!!  I am
> blocking endogenous enzymes (peroxidase etc., DAKO), avidin and biotin
> (just to see if that would help...it didn't), and protein block (it's
> literally an hour worth of blocking!!), developing with DAB (Dako) and
> hematoxylin counterstain.  I am so confused as how to get this to  
> work!
> Also, it isn't just this particular antibody it is any rabbit  
> polyclonal
> I have tried.  Could it be the polymer?  It is the one that Biocare
> suggested?  HELP!!
>
> Thanks in advance =)
>
>
>
> Sarah Goebel, BA, HT(ASCP)
>
> Histotechnologist
>
> Mirna Therapeutics
>
> 2150 Woodward Street
>
> Suite 100
>
> Austin, Texas  78744
>
> (512)901-0900 ext. 6912
>
>
>
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