[Histonet] Biopsy Run

[Yahoo]

Mesh cassettes are known to hold air pockets in them, so extending your times would only over process your tissue. 

I would suggest you use sponges, filter paper, or bx bags and use the cassettes you use for your larger specimens until you can get a different cassette. 

We found in our lab (we didn't have a printer) that the ink will fade if it's not dry enough before blocks are put in formalin container. Same on the slides. 

Hope this helps. 

Sent from the iPhone of Kim Tournear

On Apr 27, 2011, at 7:21 AM, "Marshall, Kimberly K" <kkmarshall <@t> anthc.org> wrote:

> Morning and Happy Hump day Histo peeps
> 
>  Hope everyone is having a fun filled Lab Week, Here is Alaska we are
> eating more than usual...
> 
>  Just need to ask one more question regarding my evil "nuclear
> artifact".  We have decided it is a processing issue due to the type of
> cassettes we use.  In cutting down the processing time, the reagents are
> not able to get through the mesh on the cassettes.  I have ordered other
> kinds but due to limited funds (cant get a new labeler) and even with
> the special pens for writing on cassettes and slides the hand written
> numbers are fading, I cant use them.  My next thought is to lengthen my
> biopsy run.  Would you folks out there share your biopsy run times with
> me?  I don't want to go back to having the over processing issues with
> the biopsies that occur in the 12 hour, but maybe adding time would help
> here.  
> 
> Thanks in advance and again hope everyone is having a great Lab week.
> 
> Kim
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