[Histonet] Re: processing times for skin specimens

[Tony Henwood]

This is important.
Ensure that your fixation time is as long as possible.
Short processing will give poor results if fixation is shortened

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nathan Jentsch
Sent: Friday, 22 April 2011 11:46 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: processing times for skin specimens

We do a short run some mornings for shaves and small punches.  Keep in mind that we don't work 24/7, so these have been sitting in formalin overnight.

20 min formalin
1 min distilled water
10 min 80% alcohol
10 min 90% alcohol
10 min 95% alcohol
10 min absolute alcohol
20 min absolute alcohol
10 min pro-par (xylene substitute)
10 min pro-par
20 min pro-par
10 min paraffin
10 min paraffin
10 min paraffin
10 min paraffin

That comes out to be about 3 hours of time once you factor in the pump in/pump out time, and we have a VIP 5.
Nathan Jentsch
BS HT(ASCP)
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