[Histonet] canine teeth

[kbowden]

I would decal with TBD-2 for about two weeks.  TBD-2 is a formic acid 
decal solution.  I would shake the specimen 24 hrs a day.  Vacuum and 
release the specimen several times a day.  Change the decal every couple 
of days.  Do not remove the paraffin it helps to protect from over 
decalcifying.

*//*

*/-- You are what you do - not what you say --/*

*/Karen Bowden
Staff Research Associate II
University of CA, San Diego
Department of Orthopedics
3525 John Hopkins Ct. 0863
San Diego, CA 92121-08630
858-822-1251 voice
858-534-5304 fax


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On 4/26/11 10:48 AM, Perry, Margaret wrote:
> Hi
> I was given blocks today of a canine tooth that had been decaled for 8 days.  We processed it on our regular overnight program.   It was too hard to cut and popped out of the paraffin.  The biopsy trim sheet did not indicate it was a tooth so I put the pieces in ammonium hydroxide for 10 min.  It didn't help.  Do you have any suggestion on how I can save this tissue?
>
>
> Margaret Perry HT(ASCP)
> Dept of Veterinary and  Biomedical services
> Box 2175
> South Dakota State University
> Brookings SD 57007
> 605-688-5638
>
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