[Histonet] endogenous peroxidases

[Akemi Allison]

Make sure you use a fairly fresh bottle of H2O2. (no older than 6  
months).  The stock bottle degrades after it is opened.  I have seen  
people using an older bottle of  H2O2 and the results are sub-optimal.
Akemi

Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3377 <@t> yahoo.com

On Apr 22, 2011, at 10:27 AM, Hobbs, Carl wrote:

>
> Gimme 5!
>
> Block before or after Ag retrieval.
> If it's Pwax sections make a 1/10 dilution of 100vols ( ~30%) H2O2  
> in Dist.water.
>  Block for 15mins.
> Methanol is not neccessary...imho.
> Carl
>
>
>
> Carl Hobbs
> Histology Manager
> Wolfson CARD
> School of Biomedical Sciences
> Kings College London
> Guys Campus
> London
> SE1 1UL
> Tel: 020 78486813
> Fax: 020 78486816
>
>
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