[Histonet] RE: whole retinal staining

[Abbey Mortimer]

Thank you all so very much!


Abbey Mortimer
PhD Candidate
School of Psychological Science
La Trobe University
Bundoora, Victoria, 3083
AUSTRALIA

Phone: (03) 9479 2470
Email: a.mortimer <@t> latrobe.edu.au
________________________________________
From: David A. Wright [dw18 <@t> uchicago.edu]
Sent: Wednesday, 20 April 2011 7:41 AM
To: histonet <@t> lists.utsouthwestern.edu
Cc: Abbey Mortimer
Subject: whole retinal staining

Dear Abbey & Histonet

I agree with René Buesa: I would try to stain whole retinae by a 'floating section' method i.e. hand processing in a dipping tray or mesh baskets (brush transfer can be done too but is laborious). Then mount them at the end.

Floating allows reagents to penetrate from both sides of the retina - extra valuable because your incubation times are going to be very long with material this thick. (I assume you are not just interested in superficial layers.) A microwave processor would help with penetration, but I have no direct experience.

-David
==
David A. Wright, Ph.D.
University of Chicago
Section of Neurosurgery

ORIGINAL POST:
Histonet Digest, Vol 89, Issue 21 Message: 7
Date: Tue, 19 Apr 2011 19:00:27 +1000
From: Abbey Mortimer <A.Mortimer <@t> latrobe.edu.au>
Subject: [Histonet] Tissue adhesion to slides


Hello out there!

I was wondering if any of you have experience in attaching human retinae (~250-300 microns thick) to slides for manual immunohistochemistry. Starfrost does not seem adequate.

Any advice would be greatly appreciated!
Regards,
Abbey


Abbey Mortimer
PhD Candidate
School of Psychological Science
La Trobe University
Bundoora, Victoria, 3083
AUSTRALIA

Phone: (03) 9479 2470
Email: a.mortimer <@t> latrobe.edu.au