[Histonet] IHC validation

[Rene J Buesa]

Not really because the Hematoxylin should "affect" only the nuclei UNLESS it is a nuclear antibody, in which case the antigenic reaction could be obscured. 
For nuclear Abs I used to dilute my hematoxylin as much as possible or did not use it at all.
René J.

--- On Tue, 4/19/11, Ring, Mary L <Mary.L.Ring <@t> HealthPartners.Com> wrote:


From: Ring, Mary L <Mary.L.Ring <@t> HealthPartners.Com>
Subject: [Histonet] IHC validation
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Tuesday, April 19, 2011, 3:04 PM


Does a change in the type of hematoxylin counterstain require any re-validation of IHC stains?
Thanks for your help!

Mary Ring, HT, QIHC
Regions Hospital, St Paul, Mn

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