[Histonet] Question about delayed start in Leica Tissue Processor

[Thomas, Nancy]

So that makes 4 the correct number because she is starting it on a Wednesday (day 0)  Thurs. =1,Fri.=2, Sat.=3, Sun=4 and then it would start that night at 18:20.  Sun. would be included  (according to the manual) because the count refers to how many times the clock passes by midnight.  (Wed., Thurs, Fri, and Sat. nights)
Nancy Thomas


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bascaramurty, Saro
Sent: Monday, April 18, 2011 8:33 AM
To: 'Jenny Vega'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Question about delayed start in Leica Tissue Processor

She is right.
Day 0 is today (the same day you start the run) -day 1 Day 1 is tomorrow start the run the next day,- day 2 Day 2 is the day after tomorrow - day 3 Day 3 is the following day -day 4 and so on

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jenny Vega
Sent: April 17, 2011 8:52 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Question about delayed start in Leica Tissue Processor

Hello. I have little experience using the tissue processor from my lab which is an old version of the Leica  TP1020. I was taugh that since on weekends the processor is not going to be used then you have to set up the days of delay for example 2-18:20 (2 represents saturday and sunday). On Monday
(tomorrow) I have the day off, so the processor won't be used. When I come back on Tuesday I have to change the the time as 0-18:20 so that the processor starts working that day and on wednesday. On thursday, friday, saturday and Sunday will also have the days off. I am confused because my supevisor told me to set the time as 3-18:20 next Wednesday, and since I will not be working in the lab for 4 days straight that does not make sense, it is suppose to be 4-18:20 instead. I think she made a mistake telling me that since maybe she confused the program of this week when the processor was programmed as 3-18:20 because used for 3 days straight (saturday to monday). My supervisor won't be in the lab next week so I cannot ask her.

This question seems silly but I want to be sure. I know that if tissue is left for a long time in paraffin the tissue can get over-harden and I don't want to program de delayed start as 3-18:20 thinking it is the correct way when is not.

Thanks
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