[Histonet] Sectioning undecalcified bone

[Rene J Buesa]

You will have to decalcify the bone first. Being so small pieces, I advise you to use EDTA and proceed with the regular tissue processing after the bone is decalcified.
René J.

--- On Sat, 4/16/11, awatanabe <@t> tgen.org <awatanabe <@t> tgen.org> wrote:


From: awatanabe <@t> tgen.org <awatanabe <@t> tgen.org>
Subject: [Histonet] Sectioning undecalcified bone
To: histonet <@t> lists.utsouthwestern.edu
Date: Saturday, April 16, 2011, 8:29 PM


I have a sample that is 3 needle bone biopsies that I need to get an H&E and subsequently several 12um thick sections for further assays.  The bone appears to not be decaled at all.  I don't normally have bone to cut so I'm looking for suggestions.

Aprill

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