[Histonet] Update regarding question on Plastic Embedding

[Joseph Saby]

Mahesh-

If you have access to a sonicator, you can etch the slides by first soaking them 
in 1% formic acid in the sonicator for 5-10 minutes, then after a brief water 
rinse soak them in 50% ethanol for another 5-10 minutes in the sonicator.

You may have to work with the staining times, but you will find that many of 
your paraffin embedding stains will work.

Good luck!

Joe Saby, BA HT
NAMSA



________________________________
From: Mahesh Polavarapu <polavarapu.mahesh <@t> gmail.com>
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Tue, April 12, 2011 8:25:30 PM
Subject: [Histonet] Update regarding question on Plastic Embedding

Looking for a protocol to visualize vascularization and collagen deposition
at the bone-tendon interface of a rabbit rotator cuff embedded in a plastic
system. Sections will be rather thick (~50um) b/c they are being made
through a titanium anchor. Using MMA with a cold-curing resin, Technovit
9100. Thanks in advance!

- Mahesh
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