[Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?

[Kumar, Devender]

Hi, 

We fix 'Category-A' agent infected murine lungs in 10% buffered formalin for 7 days in ABSL-3. We also confirm tissue sterility by plating the tissue homogenates on suitable media before taking the samples out of ABSL-3 facility for sectioning. In your case 7 days should be enough, but it is always better to confirm the sterility of tissue.

Thanks,
Devender


Devender Kumar, D.V.M., Ph.D.
Postdoctorate fellow,
Center for Immunology & Microbial Disease,
Albany Medical College,
47 New Scotland Avenue, MC
151
Albany, NY 12208
518.262.6220(LAB)
--


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, April 13, 2011 3:57 PM
To: histonet <@t> lists.utsouthwestern.edu; ChristinaThurby
Subject: Re: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?

5 days (96 h) is more than enough, but always handle the tissue and blocks using safety precautions.
René J.

--- On Wed, 4/13/11, Thurby, Christina <christina.thurby <@t> bms.com> wrote:


From: Thurby, Christina <christina.thurby <@t> bms.com>
Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, April 13, 2011, 2:04 PM


Hi,
Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case?  Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days.
Thanks,
Christina Thurby
Bristol-Myers Squibb Company
Research Scientist I
812-307-2093 (tie 625)




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