[Histonet] patient melanin pigment

[Patsy Ruegg]

You can remove melanin but I would think that would have an adverse effect
on IHC for sure, as Linda suggests the best bet for IHC is to use ap/red or
aec with hrp instead of DAB.  Jim Burchette has a giemsa stain he does on
skin which colors the melanin green instead of brown but I was not able to
get it to work myself.

Regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sebree Linda
A
Sent: Wednesday, April 06, 2011 12:46 PM
To: Michele Carr; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] patient melanin pigment

Are you talking about staining with H&E, special stains or immunos? If
its to stain with IHC you can use a chromogen other than DAB for your
detection kit.


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michele
Carr
Sent: Wednesday, April 06, 2011 1:16 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] patient melanin pigment

Hi, I was wondering if there were a way to remove a patients melanin
pigment on 
a slide. Any helpful advice would be appreciated.
Thanks,
Michele Carr
Medical Laboratory Services



      
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