[Histonet] RE%3A Verhoeff/Masson%27s Stain&In-Reply-To=9BF995BC0E47744E9673A4148

[Ruppert, Amysue]

Hello John,
 Just reading through some histonet archives and found your Elastic- Masson issue.
Some notes on the elastic masson stain.... The fibers should darken when the slides go into running water after the 2% ferric chloride. So the color before the ferric chloride will not be the same intense color as after the running water.  But one very important point is that with this stain, you should actually UNDER differentiate the slides in the 2% ferric chloride because the solutions in the masson part of the procedure will also differeniate out the fibers. And so you could actually wipe out the fiber staining after going through the entire procedure. 
 In our lab, the technique is to do only 2-3 dips in the 2% ferric chloride (yes, this will seem like not enough time in the ferric chloride, but remember, the masson solutions will also differentiate the slides) and then procede with the rest of the staining and then you should be seeing the fibers. 

Also, do not be afraid to try to contact PolyScientific, they are a very helpful company.
 

Good luck.
amysue ruppert

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