[Histonet] uh...

[William Chappell]

Sounds like your dehydrating alcohol is not dehydrating completely. I would switch out the 100%'s and the clearite. Either they got "wet" during the course of the day or someone made an oops when changing the stain line. 

Hope that helps. 

Will

Sent from my iPhone

On Apr 7, 2011, at 2:06 PM, <sgoebel <@t> mirnarx.com> wrote:

> So I just stained a group of slides all at the same time with the same
> conditions.  About 40 of 150 the eosin looks like it is bleeding out of
> the sections...this has never happened before?  What could be the cause
> and how do I fix it?  Everything is as normal, but again I am being
> forced to use the crappy stuff called clear rite.  Could this be the
> cause of the bleeding?
> 
> Thanks
> 
> 
> 
> Sarah Goebel, BA, HT(ASCP)
> 
> Histotechnologist
> 
> Mirna Therapeutics
> 
> 2150 Woodward Street
> 
> Suite 100
> 
> Austin, Texas  78744
> 
> (512)901-0900 ext. 6912
> 
> 
> 
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