[Histonet] patient melanin pigment

Breeden, Sara sbreeden <@t> nmda.nmsu.edu
Wed Apr 6 13:28:09 CDT 2011


Deparaffinize and hydrate to DH20.  Place slides into 0.25% potassium
permanganate for 30 min to 1 hour (visually inspect to measure level of
melanin removed); wash in running tap H20 for 1 minute and place into 5%
oxalic acid for 2-5 minutes until section becomes clear.  Wash in H20
and stain with H&E.



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