[Histonet] WNV IHC

[Jan Shivers]

Hello everyone,

A few people asked me to post some information on my West Nile Virus IHC test method, so here are some general comments.  If you'd like more detailed information, please write to me directly.

Tissue pretreatment - pressure cooker (Biocare Decloaking Chamber) with Target Retrieval solution (Dako)
Primary antibody - anti-West Nile Virus (BioReliance, cat. # 81-002; clone 7H2; working dilution at 3-6 ug/ml Ab concentration, depending upon lot)
Secondary linking antibody - EnVision+/HRP goat anti-mouse IgG (Dako; with 2-4% normal case species serum added per volume)
Chromogen - AEC+ (Dako)

Best regards,
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu

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