[Histonet] WNV IHC

Jan Shivers shive003 <@t> umn.edu
Thu Sep 30 14:52:41 CDT 2010


Hello everyone,

A few people asked me to post some information on my West Nile Virus IHC test method, so here are some general comments.  If you'd like more detailed information, please write to me directly.

Tissue pretreatment - pressure cooker (Biocare Decloaking Chamber) with Target Retrieval solution (Dako)
Primary antibody - anti-West Nile Virus (BioReliance, cat. # 81-002; clone 7H2; working dilution at 3-6 ug/ml Ab concentration, depending upon lot)
Secondary linking antibody - EnVision+/HRP goat anti-mouse IgG (Dako; with 2-4% normal case species serum added per volume)
Chromogen - AEC+ (Dako)

Best regards,
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu

(Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.)


More information about the Histonet mailing list