[Histonet] Vectastain elite ABC kit- multiple dips ok?

[Geoff McAuliffe]

Teresa Iglesias wrote:
> Hi all,
> I have three 24-well plates with three brain sections in each well. For IHC,
> do I have to prepare three different/fresh ABC dips for each 24-well screen
> plate or can I incubate (30min) each successive screen plate into the
> already used dip of ABC? 
I would not.
> Do I have to leave it in longer to allow adequate
> binding?
>   
I may be that the ABC has been consumed by the first one or two 24 well 
plates?
> I know you can do this with DAB but you have a visual indicator that you've
> left it in long enough in that case.
> Any experience with this?
>   

Why not ask Vector? After all, they made the stuff.

Geoff
> Thanks,
> -Teresa
>
>   


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff <@t> umdnj.edu
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