[Histonet] West Nile IHC

[Goins, Tresa]

After many frustrating attempts, I would greatly appreciate any information on the successful application of IHC for the detection of West Nile Virus in fixed tissue.  I have tried no retrieval, enzymatic and heat retrieval with no success on horse and bird tissues.

Would someone please share successful protocols (retrieval) and antibody sources for equine and avian fixed tissues?  Our tissues are formalin fixed and the detection method used is biotin-streptavidin, alkaline phosphatase with a new fuchsin chromogen (Vulcan Fast Red)

Thank you in advance,

Tresa Goins
Veterinary Diagnostic Lab
Department of Livestock
Bozeman, Montana