[Histonet] Necrotic and apoptotic cells

[Anatoli Gleiberman]

Anil,
For apoptotic cells in the liver you can use In situ cell death
detection kit, fluorescein (Roche, cat.#11 684 795 910) and use
counterstain with different color (Cy3, AlexaFluor 546 or Texas Red) for
the detection of endogenous IgG. Liver cells do not produce IgG, but
necrotic cells are usually impregnated with plasma proteins - and bright
staining for endogenous IgG is easy and reliable way to visualize such
cells. Works well on FFPE sections after citrate boiling retrieval.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman <@t> cbiolabs.com


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Thotakura, Anil Kumar
Sent: Thursday, September 23, 2010 2:35 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Necrotic and apoptotic cells 

Dear All,

I want to do look for Necrotic and apoptotic cells on liver tumor
sections.
What staining and antibodies are good for this technique?.

Please let me know. Thank you very much in advance.

Many Thanks,
Anil Kumar.


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