[Histonet] NFkB p65

[Anatoli Gleiberman]

Hi Fabrice,
If you are looking for NFkB activation I suggest to use antibody against
non-phosphorylated p65 (rabbit from Abcam, CHIP grade) and watch nuclear
translocation of p65. Different anti-phospho-p65 did not give me
anything, while anti-p65 clearly shows NFkB activation every time (and
it correlates very well with biochemical data). The best results I got
was with fresh-frozen sections (with subsequent formaldehyde fixation),
next - cryo sections from formaldehyde perfused mice or rats, next -
cryo from formaldehyde fixed tissues, and finally - sometimes got some
positive results from FFPE after retrieval by citrate boiling.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman <@t> cbiolabs.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Fabrice
GANKAM
Sent: Monday, September 20, 2010 6:50 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] multi-timer


Hey guys
Was wondering if any of you ever used a phopho NFKB p65 antibodu and
which
one you prefer with what antigen retrieval method if applies.
I'm working on FFPE rat tissue

Thanks.

Fabrice


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

 


This communication may contain privileged information.  It is intended solely for the use of the addressee.  If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information.  If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy.  This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act.  You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information.