[Histonet] Cutting Microarrays

[Helen Fedor]

Hello, We have trouble with the larger size punches as well. In order to get the cores to stick to the size try warming the block in a oven at 37 degrees for 15 minutes, put the block face down on a clean slide. (providing the melting temperature of the paraffin is about 57 degrees). After 15 minutes remove the block/slide complex and gently press down on the block, then allow the block to cool. Repeat the process 3 or 4 times. We have also had some success by sliding the block face over the heating unit of the paraffin embedder. But this only works for a few sections and then you get the same problem back.

Best Regards,
Helen L. Fedor 

Tissue Microarray Lab, Manager
Prostate Tissue Spore Lab, Manager
Johns Hopkins University
600 N. Wolfe St, | Marburg Room 406
Baltimore, MD | 21287-7065

410.614.1660

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of louise renton
Sent: Monday, September 20, 2010 5:51 AM
To: Rae Staskiewicz; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Cutting Microarrays

hi, this sounds like the tissue was too cool when embedded in the wax - so
it has not made a nice homogenous block that cuts as one. Can u re-embed?

On Mon, Sep 20, 2010 at 10:54 AM, Rae Staskiewicz <raestask <@t> grics.net>wrote:

>
>
> Good Morning All,
>
>
>
> I am having some issues when cutting a microarray containing 4mm punches.
> The punches are rolling and separating from the paraffin during cutting.
> Anyone have any tips or ideas??
>
>
>
> Rae Staskiewicz
>
> MMCI
>
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-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
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