[Histonet] Re: Problem with liver fixation

[Matthew Lunetta]

Itai,

Are you post fixing in Bouins? Post fix in heated Bouins for 1hr, wash well and then follow your protocal.

Hope this helps,

Matt Lunetta HT (ASCP)
Longmont United Hospital


Message: 2
Date: Wed, 15 Sep 2010 18:27:29 +0200
From: Itai Moshe 
Subject: [Histonet] Re: Problem with liver fixation
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:

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Hi Histonet's
I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with
PFA.
I'm using this protocol:
http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997
with sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079)
The staining is beautiful, but i can't see the nuclei good enough.
1) Is there a way to enhance the nuclei staining ? (the nuclei is the only
reason that i"m not using the simpler sirius red and fast green staining.)
2) What is the meaning for washing in running tap water washing, is it done
by putting the slides in a jar with simple tap water for a few minutes ?

Thank's
Itai